Summary
Seven different strains of Bacillus megaterium, obtained from strain collections, were investigated for the presence of plasmids. 6 of these contained up to four plasmids which although having identical buoyant density, could be attributed to at least 8 different size classes with contour lengths ranging from 0.63 to 8 μm. With one exception, an identity of these do not appear to the 10 previously described plasmids in strain 216 which until now was the only strain of B.megaterium to be analysed genetically. This is further indicated by the heterogeneity of the physical map of one of these plasmids to strain 216. One strain was found to contain no plasmids and thus may be used as an ideal recipient strain for molecular cloning within this species.
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References
Brown BJ, Carlton BC (1980) Plasmid-mediated transformation in Bacillus megaterium. J Bacteriol 142: 508–512
Carlton BC, Brown BJ (1979) Physical mapping of a plasmid from Bacillus megaterium by restriction endonuclease cleavage. Plasmid 2: 59–68
Hintermann G, Fischer HM, Crameri R, Hütter R (1981) Simple procedure for distinguishing CCC, OC and L forms of plasmid DNA by agarose gel electrophoresis. Plasmid 5: 371–373
Lovett PS, Keggins KM (1979) Bacillus subtilis as a host for molecular cloning. In: Wu R (ed) Methods in enzymology, vol. 68. Academic Press, New York, pp 342–356
Rehm HJ (1980) Industrielle Mikrobiologie. Springer Berlin, Heidelberg, New York, pp 353–368 and 484–505
Rostas K, Dobritsa SV, Dobritsa AP, Koncs CS, Alföldi L (1980) Megacinogenic plasmid from Bacillus megaterium 216. Molec Gen Genet 180: 323–329
Southern E (1979) Gel electrophoresis of restriction fragments. In: Wu R (ed) Methods in enzymology, vol. 68. Academic Press, New York, pp 152–175
Stahl U, Kück U, Tudzynski P, Esser K (1980) Characterization and cloning of plasmid like DNA of the ascomycete Podospora anserina. Molec Gen Genet 178: 639–646
Szybalski W (1968) Use of caesium sulfate in equilibrium density gradient centrifugation. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol. 12, Part B. Academic Press, New York, pp 330–360
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Stahl, U., Esser, K. Plasmid heterogeneity in various strains of Bacillus megaterium . European J. Appl. Microbiol. Biotechnol. 17, 248–251 (1983). https://doi.org/10.1007/BF00510424
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DOI: https://doi.org/10.1007/BF00510424