Skip to main content
SpringerLink
Log in

Regional distribution and biochemical properties of 125I-Tyr8-substance P binding sites in synaptic vesicles

  • Papers
  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Summary

  1. 1.

    Binding of 125I-Tyr8-substance P (SP) to synaptic vesicles shows an uneven distribution within the brain and the spinal cord. The regional distribution has a positive correlation with the SP-content, except in the hypothalamus.

  2. 2.

    Ca2+ and Mg2+-ions (1 and 10 mM) decrease the number of binding sites without alteration of affinity. EDTA and EGTA enhance SP-binding which is interpreted as being due to removal of the inhibitory influence of endogenous Ca2+ and Mg2+ through chelation with these agents. No significant inhibition of SP binding was observed by Na+ or K+ in concentrations below 100 mM.

  3. 3.

    Pretreatment of synaptic vesicles with trypsin or with phospholipase A2, C and D leads to a total loss of SP binding showing a proteolipid or a joint protein-phospholipid nature of these binding sites. SH groups do not contribute to SP binding since no effect of N-ethylmaleimide and monoiodoacetic acid on SP binding was found.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abood, L. G., Hoss, W. P.: Stereospecific binding of morphine to phosphatidyl serine. Psychopharmacol. Commun. 1, 29–35 (1975)

    Google Scholar 

  • Atweh, S. F., Kuhar, M. J.: Autoradiographic localization of opiate receptors in rat brain. III. The telencephalon. Brain Res. 134, 393–405 (1977)

    Google Scholar 

  • Bartlett, G. R.: Phosphorus assay in column chromatography. J. Biol. Chem. 234, 466–468 (1959)

    Google Scholar 

  • Breckenridge, W. C., Morgan, J. G., Zanetta, J. P., Vincendon, G.: Adult brain synaptic vesicles. II. Lipid composition. Biochim. Biophys. Acta 320, 681–686 (1973)

    Google Scholar 

  • Chang, M. M., Leeman, S. E.: Isolation of a sialogogic peptide from bovine hypothalamic tissue and its characterisation as substance P. J. Biol. Chem. 245, 4784–4790 (1970)

    Google Scholar 

  • Cuello, A. C., Emson, P., del Fiacco, M., Gale, J., Iversen, L. L., Jessell, T. M., Kanazawa, J., Paxions, G., Quik, M.: Distribution and release of substance P in the central nervous system. In: Centrally acting peptides (Hughes, J., ed.). London: Macmillan Press 1978

    Google Scholar 

  • De Robertis, E., Rodriguez de Lores Arneiz, G., Salganicoff, L., Pellegrino de Iraldi, A., Zieher, L. M.: Isolation of synaptic vesicles and structural organisation of the acetylcholine system within brain nerve endings. J. Neurochem. 10, 225–235 (1963)

    Google Scholar 

  • De Robertis, E., Lunt, G. S., La Torre, J. L.: Multiple binding sites for acetylcholine in a proteolipid from electric tissue. Mol. Pharmacol. 7, 97–103 (1970)

    Google Scholar 

  • Donellan, J. F., Jewess, P. J., Catell, K. J.: Subcellular localization and properties of a cholinergic receptor isolated from house fly heads. J. Neurochem. 25, 623–629 (1975)

    Google Scholar 

  • Duka, T., Höllt, V., Przewlocki, R., Wesche, D.: Distribution of methionine-and leucine-enkephalin within the rat pituitary gland by highly specific radioimmunoassays. Biochem. Biochys. Res. Commun. 85, 1119–1127 (1978)

    Google Scholar 

  • Eichberg, J., Whittaker, V. P., Dawson, R. M. C.: Distribution of lipids in subcellular particles of guinea pig brain. Biochem. J. 92, 91–100 (1964)

    Google Scholar 

  • Ellman, G. L., Courtney, K. D., Andres, Jr., V., Feathersone, R. M.: A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7, 88–95 (1961)

    Google Scholar 

  • Folch, J., Lees, M., Stanley, G. H. S.: A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509 (1957)

    Google Scholar 

  • Gamse, R., Molnar, A., Lembeck, F.: Substance P release from spinal cord slices by capsaicin. Life Sci. 25, 629–636 (1979)

    Google Scholar 

  • Glowinski, J., Iversen, L. L.: Regional studies of catecholamines in the rat brain. I. The disposition of (3H) norepinephrine, (3H) dopamine and (3H) DOPA in various regions of the brain. J. Neurochem. 13, 655–669 (1966)

    Google Scholar 

  • Hökfelt, T., Pernow, P., Nilsson, G., Wetterberg, L., Goldstein, M., Jeffcoate, S. L.: Dense plexus of substance P immunoreactive nerve terminals in eminentia medialis of the primate hypothalamus. Proc. Natl. Acad. Sci. USA 75, 1013–1015 (1978)

    Google Scholar 

  • Hosie, R. J. A.: The localization of adenosine-triphosphatases in morphologically characterized subcellular fractions of guinea pig brain. Biochem. J. 96, 404–412 (1965)

    Google Scholar 

  • Hoss, W., Okumura, K., Formaniak, M., Tanaka, R.: Relation of cation binding sites on synaptic vesicles to opiate action. Life Sci. 24, 1003–1010 (1979)

    Google Scholar 

  • Hoss, W., Smiley, C.: Immobilization of opiates by membrane lipids. Mol. Pharmacol. 13, 251–258 (1977)

    Google Scholar 

  • Inouye, A., Kataoka, K.: Sub-cellular distribution of the substance P in the nervous tissues. Nature 193, 585 (1962)

    Google Scholar 

  • Jessell, T. M., Emson, P. C., Paxinos, G., Cuello, A. C.: Topographic projections of substance P and GABA pathways in the striato-and pallido-nigral system: A biochemical and immunohistochemical study. Brain Res. 152, 487–498 (1978)

    Google Scholar 

  • Kalbhen, D. A., Koch, H. J.: Methodische Untersuchungen zur quantitativen Mikrobestimmung von ATP in biologischem Material mit dem Firefly-Enzymsystem. Z. Klin. Chem. Klin. Biochem. 5, 299–304 (1969)

    Google Scholar 

  • Lazarus, L. H., Brown, M. R., Perrin, M. H.: Distribution, localization and characteristics of neurotensin binding sites in the rat brain. Neuropharmacology 16, 625–629 (1977)

    Google Scholar 

  • Lembeck, F., Mayer, N., Schindler, G.: Substance P in rat brain synaptosomes. Naunyn Schmiedeberg's Arch. Pharmacol. 301, 17–22 (1977)

    Google Scholar 

  • Lembeck, F., Mayer, N., Schindler, G.: Substance P: Binding to lipids in the brain. Naunyn Schmiedeberg's Arch. Pharmacol. 303, 79–86 (1978)

    Google Scholar 

  • Lembeck, F., Saria, A., Mayer, N.: Substance P: Model studies of its binding to phospholipids. Naunyn Schmiedeberg's Arch. Pharmacol. 306, 189–194 (1979)

    Google Scholar 

  • Lin, H. K., Simon, E. J.: Reversal by bovine serum albumin of phospholipase A inhibition of opiate receptor binding. In: Characteristics and function of opioides (Van Ree, and Terenius, S., eds.), pp. 483–484. Amsterdam: Elsevier North Holland Biomedical Press 1978

    Google Scholar 

  • Llorente de Carlin, M. C., De Robertis, E.: Differences in the cholinergic proteolipid isolated by affinity chromatography from normal and denervated rat leg muscle. Life Sci. 19, 903–910 (1976)

    Google Scholar 

  • Loh, H. H., Cho, T. M., Wu, Y., Way, E.: Stereospecific binding of narcotics to brain cerebrosides. Life Sci. 14, 2231–2245 (1974)

    Google Scholar 

  • Loh, H. H., Law, P. Y., Ostwald, T., Cho, T. M., Way, E. L.: Possible involvement of cerebroside sulfate in opiate receptor binding. Fed. Proc. 37, 147–152 (1978)

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)

    Google Scholar 

  • Mayer, N., Lembeck, F., Saria, A., Gamse, R.: Substance P: Characteristics of binding to synaptic vesicles of rat brain. Naunyn Schmiedeberg's Arch. Pharmacol. 306, 45–51 (1979)

    Google Scholar 

  • Mroz, E. A., Brownstein, M. J., Leeman, S. E.: Distribution of immunoassayable substance P in the rat brain. Evidence for the existence of substance P-containing tracts. In: Substance P (von Euler, U. S., and Pernow, B., eds.). New York: Raven Press 1977

    Google Scholar 

  • Mudge, A. W., Leeman, S. E., Fischbach, G. D.: Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration. Proc. Natl. Acad. Sci. USA 76, 526–530 (1979)

    Google Scholar 

  • Nagy, A., Baker, R. R., Morris, S. J., Whittaker, V. P.: The preparation and characterization of synaptic vesicles of high purity. Brain Res. 169, 285–309 (1976)

    Google Scholar 

  • Nakata, Y., Kusaka, Y., Segawa, T., Yajima, H., Kitagawa, K.: Substance P: Regional distribution and specific binding to synaptic membranes in rabbit central nervous system. Life Sci. 22, 259–268 (1978)

    Google Scholar 

  • Otsuka, M., Konishi, S.: Release of substance P—like immunoreactivity from isolated spinal cord of newborn rat. Nature 264, 83–84 (1976)

    Google Scholar 

  • Pasternak, G. W., Snyder, S. H.: Opiate receptor binding: Enzymatic treatments that discriminate between agonist and antagonist interactions. Mol. Pharmacol. 11, 478–484 (1975)

    Google Scholar 

  • Pasternak, G. W., Wilson, H. A., Snyder, S. H.: Differential effects of protein modifying reagents on receptor binding of opiate agonists and antagonists. Mol. Pharmacol. 11, 340–351 (1975a)

    Google Scholar 

  • Pasternak, G. W., Snowman, A. M., Snyder, S. H.: Selective enhancement of (3H) opiate agonist binding by divalent cations. Mol. Pharmacol. 11, 735–744 (1975b)

    Google Scholar 

  • Pelletier, G., Leclerc, R., Dupont, A.: Electron microscope immunohistochemical localization of substance P in the central nervous system of the rat. J. Histochem. Cytochem. 25, 1373–1380 (1977)

    Google Scholar 

  • Pert, C. B., Snyder, S. H.: Opiate receptor binding of agonists and antagonists affected differentially by sodium. Mol. Pharmacol. 10, 686–870 (1974)

    Google Scholar 

  • Pickel, V. M., Reis, O. J., Leeman, S. E.: Ultrastructural localization of substance P in neurons in rat spinal cord. Brain Res. 122, 534–540 (1977)

    Google Scholar 

  • Ryall, R. W.: The subcellular distribution of acetylcholine, substance P, 5-hydroxytryptamine, γ-aminobutyric acid and glutamic acid in brain homogenates. J. Neurochem. 11, 131–145 (1964)

    Google Scholar 

  • Saria, A., Mayer, N., Gamse, R., Lembeck, F.: Substance P: Two different binding sites in rat brain. Proc. Eur. Soc. Neurochem. 1, 464 (1978)

    Google Scholar 

  • Schenker, C., Mroz, E. A., Leeman, S. E.: Release of substance P from isolated nerve endings. Nature 264, 790–792 (1976)

    Google Scholar 

  • Simon, E. J., Hiller, J. M.: In vitro studies of opiate receptors and their ligands. Fed. Proc. 37, 141–146 (1978)

    Google Scholar 

  • Sobel, A., Changeux, J. P.: Purification and characterization of the cholinergic receptor protein in its membrane bound and detergent soluble forms from the electric organ of torpedo marmorata. Biochem. Soc. Trans. 5, 511–514 (1977)

    Google Scholar 

  • Taylor, R. F.: Isolation and purification of cholinergic receptor proteolipids from rat gastrocnemius tissue. J. Neurochem. 31, 1183–1189 (1978)

    Google Scholar 

  • Whittaker, V. P.: Some properties of synaptic membranes isolated from the central nervous system. Ann. NY, Acad. Sci. 137, 982–998 (1966)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Experimentelle und Klinische Pharmakologie, Universitätsplatz 4, A-8010, Graz, Austria

    Alois Saria, Norbert Mayer & Fred Lembeck

  2. Institut für Histologie und Embryologie, Harrachgasse 21, A-8010, Graz, Austria

    Maria Pabst

Authors
  1. Alois Saria
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Norbert Mayer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fred Lembeck
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maria Pabst
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saria, A., Mayer, N., Lembeck, F. et al. Regional distribution and biochemical properties of 125I-Tyr8-substance P binding sites in synaptic vesicles. Naunyn-Schmiedeberg's Arch. Pharmacol. 311, 151–157 (1980). https://doi.org/10.1007/BF00510254

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00510254

Key words

Search

Navigation