Summary
A simple standard inoculation procedure has been developed to obtain growth of fungi in the form of pellets. This technique made use of filamentous mycelium from a preculture as an inoculum, yielding many small pellets with a fairly homogeneous size distribution. At an early stage of growth the presence of a polymer (Carbopol-934) proved to be very important for the way spores germinate and lowered the agglomeration tendency. At a later stage of growth the influence of shearing forces becomes more predominant.
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van Suijdam, J.C., Kossen, N.W.F. & Paul, P.G. An inoculum technique for the production of fungal pellets. European J. Appl. Microbiol. Biotechnol. 10, 211–221 (1980). https://doi.org/10.1007/BF00508608
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DOI: https://doi.org/10.1007/BF00508608