Summary
Localization of protein AP, which is known to be associated with all amyloid-laden tissues in systemic amyloidoses, was studied by an immunocytochemical peroxidase-antiperoxidase staining method in a series of localized amyloidosis, i.e. islet amyloid, lichen amyloidosus, and nodular amyloidosis of the respiratory and urinary tracts. The amyloid fibrils of these localized amyloidoses are believed to be of three different chemical classes belonging to the AE, AD and AL type, respectively. The present study revealed that protein AP was present in amyloid deposits in all tissues examined, thus supporting that protein AP is present in amyloid deposits not only of all types of systemic amyloidosis but also different forms of localized amyloidosis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benditt EP, Cohen AS, Costa PP, Franklin EC, Glenner GG, Husby G, Natvig JB, Mandema E, Osserman EF, Sohar E, Wegelius O, Westermark P (1980) Guidelines for nomenclature. In: Glenner GG, Costa PP, Freitas F (eds) Amyloid and amyloidosis. Excerpta Medica, Amsterdam, pp XI-XII
Bonar L, Cohen AS, Skinner M (1969) Characterization of the amyloid fibril as a cross-β-protein. Proc Soc Exp Biol Med 131:1373–1375
Cathcart ES, Skinner M, Cohen AS (1971) Immunogenicity of amyloid. Immunology 20:945–954
Cohen AS, Wegelius O (1980) Classification of amyloid: 1979–1980. Arthritis Rheum 23:644–645
Page DL, Isersky C, Harada M, Glenner GG (1972) Immunoglobulin origin of localized nodular pulmonary amyloidosis. Res Exp Med 159:75–86
Pearse AGE, Ewen SWB, Polak JM (1972) The genesis of apudamyloid in endocrine polypeptide tumours: histochemical distinction from immunamyloid. Virchows Arch B 10:93–107
Pepys MB, Dash AC, Munn EA, Feinstein A, Skinner M, Cohen AS, Gewurz H, Osmand AP, Painter RM (1977) Isolation of amyloid P-component (protein AP) from normal serum as a calcium-dependent binding protein. Lancet 1:1029–1031
Skinner M, Pepys M, Cohen AS, Heller LM, Lian JB (1980) Studies on amyloid protein AP. In: Glenner GG, Costa PP, Freitas F (eds) Amyloid and amyloidosis. Excerpta Medica, Amsterdam, pp 384–391
Sletten K, Westermark P, Natvig JB (1976) Characterization of amyloid fibril proteins from medullary carcinoma of the thyroid. J Exp Med 143:993–998
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method for immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Termine JD, Eanes ED, Ein D, Glenner GG (1972) Infrared spectroscopy of human amyloid fibrils and immunoglobulin proteins. Biopolymers 2:1103–1113
Thompson AR, Enfield DL (1978) Human plasma P component: Isolation and characterization. Biochemistry 17:4304–4311
Westermark P, Stenkvist B (1973) A new method for the diagnosis of systemic amyloidosis. Arch Intern Med 132:522–523
Westermark P, Skinner M, Cohen AS (1975) The P-component of amyloid of human islets of Langerhans. Scand J Immunol 4:95–97
Westermark P, Sletten K, Pitkänen P, Natvig JB, Lindholm C-E (1981) Localized laryngeal amyloidosis: partial characterization of an amyloid fibril protein AL. (In press)
Author information
Authors and Affiliations
Additional information
Supported by the Swedish Medical Research Council (Project No. B81-12X-05941-01), the Research Fund of King Gustaf V and grants from the United States Public Health Service, National Institute of Arthritis, Metabolism and Digestive Diseases (AM 04599 and AM 07014), National Institute of Health Multipurpose Arthritis Center (AM 20613), from the General Clinical Research Centers Branch of the Division of Research Resources, National Institutes of Health (RR 533), from the Massachusetts Chapter of the Arthritis Foundation and from the Arthritis Foundation
Rights and permissions
About this article
Cite this article
Westermark, P., Shirahama, T., Skinner, M. et al. Amyloid P-component (protein AP) in localized amyloidosis as revealed by an immunocytochemical method. Histochemistry 71, 171–175 (1981). https://doi.org/10.1007/BF00507821
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00507821