Skip to main content
SpringerLink
Log in

GABA transport in the rat thyroid

  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Summary

  1. 1.

    The uptake of γ-aminobutyric acid (GABA) into rat thyroid slices was studied.

  2. 2.

    Uptake of 14C-GABA was concentration-dependent: one unsaturable (diffusion) and two saturable components obeying Michaelis-Menten kinetics contributed to transport.

  3. 3.

    The kinetic constants of saturable GABA transport systems were: K m1 =1.5 μM, V 1=4.0 nmol×(g wet weight)−1×(20 min)−1 (high-affinity uptake); K m2 =800 μM, V 2=260 nmol×(g wet weight)−1×(20 min)−1 (low-affinity uptake).

  4. 4.

    Uptake mediated by each of the carrier systems was concentrative, entirely Na+-dependent, and required activation energies characteristic for active transport.

  5. 5.

    High-affinity transport was structurally specific for GABA. The substrate specificity of low-affinity uptake resembled that of β-amino acid transport systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Balázs R, Machiyama Y, Hammond BJ, Julian T, Richter D (1970) The operation of the γ-aminobutyrate bypath of the tricarboxylic acid cycle. Biochem J 116:445–467

    Google Scholar 

  • Blaustein MP, King AC (1976) Influence of membrane potential on the sodium-dependent uptake of gamma-aminobutyric acid by presynaptic nerve terminals: Experimental observations and theoretical considerations. J Membr Biol 30:153–173

    Google Scholar 

  • Böhlen P, Huot S, Palfreyman MG (1979) The relationship between GABA concentrations in brain and cerebrospinal fluid. Brain Res 167:297–305

    Google Scholar 

  • Bowery NG, Neal MJ (1978) cic-3-Aminocyclohexane carboxylic acid, a selective inhibitor and substrate for the neuronal GABA uptake process. In: Fonnum F (ed) Amino acids as chemical transmitters. Plenum Press, New York, pp 351–356

    Google Scholar 

  • Christensen HN (1964) Relations in the transport of β-alanine and the α-amino acids in the Ehrlich cell. J Biol Chem 239:3584–3589

    Google Scholar 

  • Crailsheim K, Gebauer H (1980) Glutamate decarboxylase in the rat thyroid gland. In: Brzin M, Sket M, Bachelard H (eds) Synaptic constituents in health and disease. Pergamon Press, Oxford, p 176

    Google Scholar 

  • Curtis DR (1979) Gabaergic transmission in the mammalian central nervous system. In: Krogsgaard-Larsen P, Scheel-Krüger J, Kofod H (eds) GABA-neurotransmitters. Munksgaard, Copehagen, pp 17–27

    Google Scholar 

  • Cutler RWP, Young J (1979) Bidirectional movement of γ-aminobutyric acid in cerebral cortex slices incubated in small volumes of medium. Brain Res 165:261–270

    Google Scholar 

  • DeFeudis FV, Ossola L, Elkouby A, Wolff P, Mandel P (1979) Effects of β-alanine, l-2,4-diaminobutyric acid and nipecotic acid on the sodium-dependent binding of (3H)GABA to brain particles. Gen Pharmacol 10:423–426

    Google Scholar 

  • East JM, Dutton GR, Currie DN (1980) Transport of GABA, β-alanine and glutamate into perikarya of postnatal rat cerebellum. J Neurochem 34:523–530

    Google Scholar 

  • Fahn S (1976) Regional distribution studies of GABA and other putative neurotransmitters and their enzymes. In: Roberts E, Chase TN, Tower DB (eds) GABA in nervous system function. Raven Press, New York, pp 169–186

    Google Scholar 

  • Fehlmann M, LeCam A, Kitabgi P, Rey JF, Freychet P (1979) Regulation of amino acid transport in the liver. Emergence of a high affinity transport system in isolated hepatocytes from fasting. J Biol Chem 254:401–407

    Google Scholar 

  • Gebauer H (1979) Multiple transport systems for neurotransmitter amino acids in the rat thyroid. Acta endocr (Kbh) Suppl 225:1

    Google Scholar 

  • Gebauer H, Haas K (1980) γ-Aminobutyric acid transport in thyroids of triiodothyronine- and propylthiouracil-treated rats. Hoppe-Seyler's Z Physiol Chem 361:1284

    Google Scholar 

  • Gebauer H, Hagmüller K (1977) The uptake of 14C-γ-aminobutyric acid into various organs of the rat. Zool Jb Physiol 81:335–342

    Google Scholar 

  • Hammerman M, Sacktor B (1978) Transport of β-alanine in renal brush border membrane vesicles. Biochem Biophys Acta 509:338–347

    Google Scholar 

  • Hertz L, Wu PH, Schousboe A (1978) Evidence for net uptake of GABA into mouse astrocytes in primary cultures. Its sodium dependence and potassium independence. Neurochem Res 3:313–323

    Google Scholar 

  • Hofstee BHJ (1952) On the evaluation of the constants V m and K M in enzyme reactions. Science 116:329–331

    Google Scholar 

  • Holzer P, Hagmüller K (1979) Transient apnoea after systemic injection of GABA in the rat. Naunyn-Schmiedeberg's Arch Pharmacol 308:55–60

    Google Scholar 

  • Hruska RE, Padjen A, Bressler R, Yamamura HI (1978) Taurine: Sodium-dependent, high-affinity transport into rat brain synaptosomes. Mol Pharmacol 14:77–85

    Google Scholar 

  • Iversen LL, Kelly JS (1975) Uptake and metabolism of γ-amino-butyric acid by neurones and glial cells. Biochem Pharmacol 24:933–938

    Google Scholar 

  • Kletzien RF, Perdue JF (1974) Sugar transport in chick embryo fibroblasts. J Biol Chem 249:3366–3374

    Google Scholar 

  • Kulakowski EC, Maturo J, Schaffer SW (1978) The identification of taurine receptors from rat heart sarcolemma. Biochem Biophys Res Comm 80:936–941

    Google Scholar 

  • Lähdesmäki P, Oja SS (1973) On the mechanism of taurine transport at brain cell membranes. J Neurochem 20:1411–1417

    Google Scholar 

  • Larsson OM, Krogsgaard-Larsen P, Schousboe A (1980) High-affinity uptake of (RS)-nipecotic acid in astrocytes cultured from mouse brain. Comparison with GABA transport. J Neurochem 34:970–977

    Google Scholar 

  • Levi G, Raiteri M (1974) Exchange of neurotransmitter amino acid at nerve endings can simulate high affinity uptake. Nature (Lond) 250:735–737

    Google Scholar 

  • Mark J, Borg J, Ramaharobandro N, Mandel P (1979) Cellular maturation and GABA uptake by neuronal and glial primary cultures. In: Mandel P, DeFeudis FV (eds) GABA—biochemistry and CNS functions. Plenum Press, New York, pp 239–250

    Google Scholar 

  • Martin DL (1973) Kinetics of the sodium-dependent transport of gamma-aminobutyric acid by synaptosomes. J Neurochem 21:345–356

    Google Scholar 

  • Martin DL (1976) Carrier-mediated transport and removal of GABA from synaptic regions. In: Roberts E, Chase TN, Tower DB (eds) GABA in nervous system function. Raven Press, New York, pp 347–386

    Google Scholar 

  • Martin DL, Shain W (1979) High affinity transport of taurine and β-alanine and low-affinity transport of γ-aminobutyric acid by a single transport system in cultured glioma cells. J Biol Chem 254:7076–7084

    Google Scholar 

  • Musashi A (1954) Synthese der Gamma-Aminocrotonsäure und der Gamma-Aminobuttersäure. Hoppe-Seyler's Z Physiol Chem 297:71–73

    Google Scholar 

  • Oja SS, Kontro P, Lähdesmäki P (1977) Amino acids as inhibitory neurotransmitters. Prog Pharmacol 1:1–119

    Google Scholar 

  • Roberts PJ (1976) Gamma-aminobutyric acid homoexchange in sensory ganglia. Brain Res 113:206–209

    Google Scholar 

  • Roskoski R (1978) Net uptake of l-glutamate and GABA by high-affinity synaptosomal transport systems. J Neurochem 31:493–498

    Google Scholar 

  • Rozen R, Tenenhouse HS, Scriver CR (1979) Taurine transport in renal brush-border membrane vesicles. Biochem J 180:245–248

    Google Scholar 

  • Schousboe A (1979) Effects of GABA-analogues on the high-affinity uptake of GABA in astrocytes in primary cultures. In: Mandel P, DeFeudis FV (eds) GABA—biochemistry and CNS functions. Plenum Press, New York, pp 219–237

    Google Scholar 

  • Schousboc A, Hertz L, Svenneby G (1977) Uptake and metabolism of GABA in astroyctes cultured from dissociated mouse brain hemispheres. Neurochem Res 2:217–229

    Google Scholar 

  • Schousboc A, Krogsgaard-Larsen P, Svenneby G, Hertz L (1978) Inhibition of the high-affinity, net uptake of GABA into cultured astrocytes by β-proline, nipecotic acid and other compounds. Brain Res 153:623–626

    Google Scholar 

  • Schrier BK, Thompson EJ (1974) On the role of glial cells in the mammalian nervous system. J Biol Chem 249:1769–1780

    Google Scholar 

  • Sellström A, Henn F (1976) Role of exchange in ‘high-affinity’ amino acid neurotransmitter uptake. Trans Am Soc Neurochem 233

  • Shank RP, Aprison MH (1979) Biochemical aspects of the neurotransmitter function of glutamate. In: Filer LJ, Garattini S, Kare MR, Reynolds WA, Wurtman RJ (eds) Glutamic acid: advances in biochemistry and physiology. Raven Press, New York, pp 139–150

    Google Scholar 

  • Taniguchi H, Okada Y, Seguchi H, Shimada C, Seki M, Tsutou A, Baba S (1979) High concentration of gamma-aminobutyric acid in pancreatic beta cells. Diabetes 28:629–633

    Google Scholar 

  • Taniguchi H, Okada Y, Shimada C, Baba S (1977) GABA in pancreatic islets. Arch Histol Japon 40 Suppl:87–97

    Google Scholar 

  • Welty JD, McBroom MJ, Appelt AW, Peterson MB, Read WO (1976) Effects of taurine on heart and brain electrolyte imbalances. In: Huxtable R, Barbeau A (eds) Taurine. Raven Press, New York, pp 155–163

    Google Scholar 

  • Wheeler DD, Hollingsworth RG (1979) A model of GABA transport by cortical synaptosomes from the Long-Evans rat. J Neurosci Res 4:265–289

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Zoologie (Tierphysiologie) der Universität Graz, Universitätsplatz 2, A-8010, Graz, Austria

    Hans Gebauer

Authors
  1. Hans Gebauer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gebauer, H. GABA transport in the rat thyroid. Naunyn-Schmiedeberg's Arch. Pharmacol. 317, 61–66 (1981). https://doi.org/10.1007/BF00506258

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00506258

Key words

Search

Navigation