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On-line estimation of cell growth for glutamic acid fermentation system

  • Biotechnology
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Summary

For glutamic acid fermentation system using molasses, on-line estimation of cell growth during the exponential growth phase was investigated based on the measurement of carbon dioxide evolution rate (CER). The CER was continuously calculated using microcomputer together with O2 and CO2 analyzers. It was found that a linear relationship existed between CER and cell concentration. Therefore it was possible to determine specific growth rate and specific CER accurately by measuring CER with time.

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Abbreviations

CER:

carbon dioxide evolution rate [mmole·l−1·min−1

F N :

nitrogen flow rate [mmole·min−1

N :

tora number of sampling

PO 2 :

mole fraction of oxygen in exit gas

PCO 2 :

mole fraction of carbon dioxide in exit gas

q CO 2 :

specific carbon dioxide evolution rate [mmole·g−1·min−1]

q CO 2 :

average specific carbon dioxide evolution rate [mmole·g−1·min−1]

ttime [h]:

V liquid volume [l]

X :

cell concentration [g·l−1]

n :

number of sampling

δ:

difference over a small time interval

μ:

specific growth rate [h−1]

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Authors and Affiliations

  1. Foods R & D Center, Cheil Sugar Co., Ltd., 92 Gayang-Dong, Kangseo-Ku, Seoul, Korea

    Sun Ho Park, Ki Tae Hong, Jae Heung Lee & Jong Chan Bae

Authors
  1. Sun Ho Park
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  2. Ki Tae Hong
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  3. Jae Heung Lee
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  4. Jong Chan Bae
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Park, S.H., Hong, K.T., Lee, J.H. et al. On-line estimation of cell growth for glutamic acid fermentation system. European J. Appl. Microbiol. Biotechnol. 17, 168–172 (1983). https://doi.org/10.1007/BF00505883

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  • DOI: https://doi.org/10.1007/BF00505883

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