Summary
Besides apamin, the structurally related MCD peptide (mast cell degranulating peptide; peptide 401) is another centrally acting peptide from bee venom. In contrast to apamin, it is hardly neurotoxic upon intravenous injection in mice. Following intraventricular injection, as little as 0.3 μg/animal produce convulsions and respiratory arrest in mice. The clinical picture differs from that elicited by apamin, and apamin is about 10 times more potent than MCD peptide when given intraventricularly.
Apamin and MCD peptide, injected into the spinal cord of rats in nanogram amounts, produce circumscript hyperexcitation lasting more than one day, however with complete recovery following sublethal doses. Local apamin poisoning differs from local tetanus (elicited by the same way) by its faster time course.
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References
Billingham, M. E. J., Morley, J., Hanson, J. M., Shipolini, R. A., Vernon, C. A.: An anti-inflammatory peptide from bee venom. Nature 245, 163–164 (1973)
Breithaupt, H., Habermann, E.: Mastzelldegranulierendes Peptid (MCD-Peptid) aus Bienengift: Isolierung, biochemische und pharmakologische Eigenschaften. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 261, 252–270 (1968)
Callewaert, G. L., Shipolini, R. A., Vernon, C. A.: The disulphide bridges of anamin. FEBS Letters 1, 111–113 (1968)
Habermann, E.: Bee and wasp venoms. Science 177, 314–322 (1972)
Habermann, E., Cheng-Raude, D.: Central neurotoxicity of apamin, crotamin, phospholipase A and α-amanitin. Toxicon 13, 465–473 (1975)
Habermann, E., Reiz, K. G.: Ein neues Verfahren zur Gewinnung der Komponenten von Bienengift, insbesondere des zentralwirksamen Peptids Apamin. Biochem. Z. 341, 451–466 (1965)
Haley, T. J., McCormick, W. G.: Pharmacological effects produced by intracerebral injection of drugs in the conscious mouse. Br. J. Pharmacol. 12, 12 (1957)
Haux, P.: Die Aminosäurensequenz von MCD-Peptid, einem spezifisch mastzell-degranulierenden Peptid aus Bienengift. Hoppe-Seylers Z. Physiol. Chem. 350, 536–546 (1969)
Haux, P., Sawerthal, H., Habermann, E.: Sequenzanalyse des Bienengift-Neurotoxins (Apamin) aus seinen tryptischen und chymotryptischen Spaltstücken. Hoppe-Seylers Z. Physiol. Chem. 348, 737–738 (1967)
Shipolini, R. A., Bradbury, A. F., Callewaert, G. L., Vernon, C. A.: The structure of apamin. Chem. Comm. 1967, 679–680
Spoerri, P. E., Jentsch, J., Glees, P.: Apamin from bee venom: Effects of the neurotoxin on cultures of the embryonic mouse cortex. Neurobiol. 3, 207–214 (1973)
Spoerri, P. E., Jentsch, J., Glees, P.: Apamin from bee venom: Effects of the neurotoxin on subcellular particles of neural cultures. FEBS Letters 53, 143–147 (1975)
Vernon, C. A., Hanson, J. M., Brimblecombe, R. W.: Br. Pat. No. 1314823 (1969)
Wellhöner, H. H.: Spinale Wirkungen von Apamin. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 262, 29–41 (1969)
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Habermann, E. Neurotoicity of apamin and MCD peptide upon central application. Naunyn-Schmiedeberg's Arch. Pharmacol. 300, 189–191 (1977). https://doi.org/10.1007/BF00505050
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DOI: https://doi.org/10.1007/BF00505050