Summary
The lipids of two strains of Lipomyces kononenkoae, grown in batch culture, were extracted and analysed. The major lipids present were phospholipids, free sterols, esterified sterols, and triacylglycerols. Phospholipid analysis indicated that phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol were the major ones. The fatty-acyl residues were C12-C18 and contained 67–74% unsaturated residues. Polyunsaturated residues accounted for 15% and 30% in L. kononenkoae CBS 2514 and L. kononenkoae CBS 5608, respectively. Analysis of the fatty-acyl residues of a low-density vesicle fraction obtained from sphaeroplasts of L. kononenkoae CBS 2514 was carried out and the results are discussed in relation to plasma membrane synthesis. The suitability of L. kononenkoae for production of single-cell protein is also discussed.
Similar content being viewed by others
References
Alterthum, F., Rose, A.H. (1973). J. Gen. Microbiol. 77, 371–382
Cartledge, T.G., Rose, A.H., Belk, D.M., Goodall, A.A. (1977). J. Bacteriol. 132, 426–433
Clausen, M.K., Christiansen, K., Jensen, P.K., Behnke, O. (1974). FEBS Lett. 43, 176–179
Erwin, J.A. (1973). Lipids and biomembranes of eukaryotic micro-organisms. New York: Academic
Haley, J.E., Jack, R.C. (1974). Lipids 9, 679–681
Hossack, J.A., Rose, A.H. (1976). J. Bacteriol. 127, 67–75
Hossack, J.A., Belk, D.M., Rose, A.H. (1977). Arch. Microbiol. 114, 137–142
Hossack, J.A., Wheeler, G.E., Rose, A.H. (1973). Fragility of plasma membranes in Saccbaromyces cerevisiae. In: Yeast, mould and plant protoplasts, J.R. Villanueva, I. Garcia-Acha, S. Gascón, F. Uruburu, eds., pp. 211–227. London: Academic
Hunter, K., Rose, A.H. (1971). Yeast lipids and membranes. In: The yeasts, Vol. 2, A.H. Rose, J.S. Harrison, eds., pp. 211–270. London: Academic
Hunter, K., Rose, A.H. (1972). Biochim. Biophys. Acta (Amst.) 260, 639–653
Kitamura, K., Kaneko, T., Yamamoto, Y. (1971). Arch. Biochem. Biophys. 145, 402–404
Kitamura, K., Kaneko, T., Yamamoto, Y. (1972). J. Gen. Appl. Microbiol. 18, 57–71
Lodder, J. (1970). The yeasts, a taxonomic study. Amsterdam: North-Holland
Pitryuk, I.A., Zvyagintseva, I.S., Bab'eva, I.P. (1975). Mikrobiologiya 44, 813–819
Protein Advisory Group of the United Nations (1975). PAG Guideline for preclinical testing of novel sources of protein. In: Single-cell Protein, Vol. 11, S.R. Tannenbaum, D.I.C. Wang, eds., pp. 629–654. Cambridge, Massachussets: MIT Press
Rattray, J.B.M., Schibeci, A., Kidby, D.K. (1975). Bact. Rev. 39, 197–231
Spencer-Martins, I., van Uden, N. (1977). Eur. J. Appl. Microbiol. 4, 29–35
Steiner, M.R., Lester, R.L. (1972). Biochim. Biophys. Acta (Amst.) 260, 222–243
Suzuki, T., Hasegawa, K. (1974a). Agr. Biol. Chem. 38, 1371–1376
Suzuki, T., Hasegawa, K. (1974b). Agr. Biol. Chem. 38, 2269–2271
Uzuka, Y., Kanamori, T., Koga, T., Tanaka, K., Naganuma, T. (1975). J. Gen. Appl. Microbiol. 21, 157–168
Uden, N. van (1967). Arch. Mikrobiol. 58, 155–168
Wickerham, L.J. (1951). Taxonomy of yeasts. 1. Techniques of classification. US Dept. Agric. Tech. Bull, No. 1029. US Dept. Agric. Washington D.C.
Zvyagintseva, I.S., Pitryuk, I.A., Bab'eva, I.P., Ruban, E.L. (1975). Mikrobiologiya 44, 625–631
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hossack, J.A., Spencer-Martins, I. Lipid composition of the yeast. European J. Appl. Microbiol. Biotechnol. 5, 273–278 (1978). https://doi.org/10.1007/BF00504715
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00504715