Summary
Synthesis and release of 3H-DA were examined during the continuous superfusion of a limited area of the ventricular surface of the cat caudate nucleus with l-3,5-3H-tyrosine, using a cup technique. 3H−H2O, an index of the conversion of l-3,5-3H-tyrosine into 3H-Dopa, and 2H-DA were estimated in serial superfusate fractions. Alpha-methyl-paratyrosine (α-MpT) (10−4 M) inhibited rapidly and simultaneously both 3H−H2O formation and 3H-DA release. Transection of the nigro-striatal dopaminergic pathway immediately blocked 3H-DA release but 3H−H2O levels were reduced by 30% one hour later.
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References
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Besson, M.J., Cheramy, A., Gauchy, C. et al. In vivo continuous estimation of 3H-dopamine synthesis and release in the cat caudate nucleus. Naunyn-Schmiedeberg's Arch. Pharmacol. 278, 101–105 (1973). https://doi.org/10.1007/BF00501867
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DOI: https://doi.org/10.1007/BF00501867