Summary
Application of filipin to sympathetic ganglia results in membrane deformations in both the neurons and the satellite cells. The plasma membranes of the principal ganglion cells show a non-homogeneous distribution of filipin induced deformations with fewer deformations in the perikaryal plasma membrane than in the nerve fiber membrane. The filipin induced membrane lesions are correlated to the number of IMPs of the neuronal membrane i.e. a high density of intramembrane particles (IMP) gives fewer deformations and vice versa. The membrane of the satellite cells contain a higher density of probe induced lesions than the neuronal membrane. The filipin induced deformations in the satellite cells are not correlated to the number of IMPs or to the number of orthogonal arrays of small particles (OAP). Specialized membrane areas such as the gap junction is always devoided of filipin induced lesions. A similar distribution of membrane lesions was found when tomatin was used instead of filipin. These results indicate a possible difference in lipid content between various parts of the neurons and between the neuronal and satellite cell plasma membrane in guinea pig sympathetic ganglia.
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Alroy JF, Merk FB, Goyal V, Ucci A (1981) Heterogeneous distribution of filipin-sterol complexes in nuclear membranes. Biochim Biophys Acta 649:239–243
Andersson Forsman C (1984) Freeze-fracture cytochemistry of sympathetic ganglia: Distribution of filipin induced membrane deformations in neurons and satellite cells. Proc Eur Cong Electron Microsc 3:1769–1770
Andersson Forsman C, Elfvin LG (1983) An ultrastructural study of presynaptic membrane specializations in sympathetic ganglia of 4-aminopyridine treated guinea pigs and rats. Brain Res 280:355–360
Andrews LD, Cohen AI (1981) Freeze-fracture evidence for the presence of cholesterol particle-free patches of basal discs and plasma membrane of retinal and rod outer segments of mice and frogs. J Cell Biol 81:215–228
Andrews LD, Cohen AI (1983) Freeze-fracture studies of photoreceptor membranes: New observations bearing upon the distribution of cholesterol. J Cell Biol 97:749–755
Bittman R, Rottem S (1976) Distribution of cholesterol between the outer and inner halves of the lipid bilayer of mycoplasma cell membranes. Biochem Biophys Res Commun 71:318–324
Bridgman PC, Nakajima Y (1981) Membrane lipid heterogeneity associated with acetylcholine receptor particle aggregates in Xenopus embryonic muscle cells. Proc Natl Acad Sci USA 78:1278–1282
Bridgman PC, Nakajima Y (1983) Distribution of filipin-sterol complexes on cultured muscle cells: cell-substratum contact areas associated with acetylcholine receptor clusters. J Cell Biol 96:363–372
Dermietzel R (1973) Visualization by freze-fracturing of regular structure in glial cell membranes. Naturwissenschaften 60:208
Elfvin LG (1961) Electron microscopic investigation of the plasma membrane and myelin sheath of autonomic nerve fibers in the cat. J Ultrastruct Res 5:388–407
Elfvin LG (1962) Electron microscopic studies on the effect of anisotonic solutions on the structure of unmyelinated splenic nerve fibers of the cat. J Ultrastruct Res 7:1–38
Elfvin LG (1963) The ultrastructure of the superior cervical sympathetic ganglion of the cat. I. The structure of the ganglion cell processes as studied by serial sections. J Ultrastruct Res 8:403–440
Elfvin LG, Forsman C (1978) The ultrastructure of junctions between satellite cells in mammalian sympathetic ganglia as revealed by freeze-etching. J Ultrastruct Res 63:261–274
Elias PM, Friend DS, Goerke J (1979) Membrane sterol heterogeneity. Freeze-fracture detection with saponins and filipin. J Histochem Cytochem 27:1247–1260
Fischer KA (1976) Analysis of membrane halves: cholesterol. Proc Natl Acad Sci USA 73:173–177
Friend DS, Bearer EL (1981) β-hydroxysterol distribution as determined by freeze-fracture cytochemistry. Histochem J 13:535–546
Garcia-Segura LM, Baetens D, Orci L (1982) Freeze-fracture cytochemistry of neuronal membranes: inhomogeneous distribution of filipin-sterol complexes in perikarya, dendrites and axons. Brain Res 234:494–499
Gotow T (1984) Cytochemical characteristics of astrocytic plasma membranes specialized with numerous orthogonal arrays. J Neurocytol 13:431–448
Gotow T, Hashimoto PH (1983) Regional difference in effect of filipin in plasma membranes of epithelial cells and surrouding free cells in the choroid plexus. Cell Tissue Res 230:689–694
Hendersson D, Eibl H, Weber K (1979) Structure and biochemistry of mouse hepatic gap junctions. J Mol Biol 132:193–218
Jain MK (1975) Role of cholesterol in biomembranes and related systems. Curr Top Membr Transp 6:1–57
Korn ED (1969) Cell membranes: structure and synthesis. Annu Rev Biochem 38:263–322
Kruijff B de, Demel RA (1974) Polyene-sterol interactions in membranes of Acholeplasma laidlawii cells and lecithin membranes III. Molecular structure of the polyene antibiotic-cholesterol complexes. Biochim Biophys Acta 339:57–70
Landis DMD, Reese TS (1974) Arrays of particles in freeze-fractured astrocytic membranes. J Cell Biol 60:316–320
Landis DMD, Reese TS (1982) Regional organization of astrocytic membranes in cerebellum cortex. Neuroscience 7:937–950
Montesano R (1979) Inhomogeneous distribution of filipin-sterol complexes in smooth muscle cell plasma membrane. Nature 280:328–329
Nakajima Y, Bridgman PC (1981) Absence of filipin-sterol complexes from the membranes of active zones and acetylcholine receptor aggregates at frog neuromuscular junctions. J Cell Biol 88:453–458
Nicolson GL (1976) Transmembrane control of the receptors on normal and tumor cells. I Cytoplasmic infuence over cell surface components. Biochim Biophys Acta 457:57–108
Orci L, Perrelet A, Montesano R (1983) Differential filipin labeling of the luminal membranes lining the pancreatic acinus. J Histochem Cytochem 31:952–955
Orci L, Singh A, Amherdt M, Brown D, Perrelet A (1981) Microheterogeneity of protein and sterol content in kidney podocyte membrane. Nature 293:646
Pearse BMF (1975) Coated vesicles from pig brain: purification and biochemical characteristics. J Mol Biol 97:93–98
Perrelet A, Garcia-Segura LM, Singh A, Orci L (1982) Distribution of cytochemically detectable cholesterol in electric organ of torpedo marmorata. Proc Natl Acad Sci USA 79:2598–2602
Pfenninger KH, Bunge RP (1975) Freeze-fracturing of nerve growth cones and young fibers. A study of developing plasma membranes. J Cell Biol 53:180–196
Pumplin DW, Bloch RJ (1983) Lipid domains of acetylcholine receptor clusters detected with saponin and filipin. J Cell Biol 97:1043–1054
Robenek H, Jung W, Gebhardt R (1982) The topography of filipin-cholesterol complexes in the plasma membrane of cultured hepatocytes and their relation to cell junction formation. J Ultrastruc Res 78:95–106
Robinson JM, Karnovsky MJ (1980) Evaluation of the polyene antibiotic filipin as a cytochemical probe for cholesterol. J Histochem Cytochem 28:161–168
Severs NJ (1981) Locations of cholesterol in the Golgi apparatus of cardiac muscle cells. Experientia 37:1195–1198
Severs NJ, Robenek H (1983) Detections of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry. Biochim Biophys Acta 737:373–408
Severs NJ, Simons HL (1983) Failure of filipin to detect cholesterol-rich domains in smooth muscle plasma membrane. Nature 303:637–638
Stubbs CD, Ketterer B, Hicks RM (1979) The isolation and analysis of the luminal plasma membrane of calf urinary bladder epithelium. biochim Biophys Acta 588:58–72
Tillack TW, Kinsky SC (1973) A freeze-etch study of the effects of filipin on liposomes and human erythrocyte membranes. Biochim Biophys Acta 323:43–54
Verkleij AJ, De Kruijff JB, Gerritsen WF, Demel RA, Van Deenen LLM, Ververgaert PHJ (1973) Freeze-etch electron microscopy of erythrocytes, Acholeplasma laidlawii cells and liposomal membranes after action of filipin and amphotericin B. Biochim Biophys Acta 291:577–581
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Andersson Forsman, C. Freeze-fracture cytochemistry of sympathetic ganglia. Histochemistry 82, 209–218 (1985). https://doi.org/10.1007/BF00501397
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DOI: https://doi.org/10.1007/BF00501397