Summary
Protoplasts of auxotrophic mutants of the highly fermentative Saccharomyces cerevisiae and the osmotolerant S. mellis were fused. The frequency of appearance of prototrophic hybrids was 0.75–1.6 per 105 protoplasts. Biochemical analysis of the stable hybrids revealed the characteristics of both parents in about 50% of the recombinants. Fermentation at high glucose concentrations by some of the hybrids was considerably improved.
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Legmann, R., Margalith, P. Interspecific protoplast fusion of Saccharomyces cerevisiae and Saccharomyces mellis . European J. Appl. Microbiol. Biotechnol. 18, 320–322 (1983). https://doi.org/10.1007/BF00500499
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DOI: https://doi.org/10.1007/BF00500499