Abstract
Carbonic anhydrase III (CAIII), an enzyme recently shown by conventional electrophoresis to be muscle specific, has been quantitated by “rocket” immunoelectrophoresis. This more sensitive technique has shown that the enzyme is virtually specific to skeletal muscle, where it occurs at a level of 5mg/g, with trace levels in smooth muscle, cardiac muscle, and lung. In man there does not appear to by any correlation between CAIII levels and the proportion of red and white muscle fibers. The fetal development of CAIII has also been examined using immunoelectrophoresis, and the enzyme can be detected at 11 weeks' gestation. The CAIII level rises gradually up to 25 weeks, and there is then a more dramatic increase to reach approximately half adult level at birth.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Carter, N., Shiels, A., and Tashian, R. E. (1978). Carbonic anhydrase III isozyme from human and bovine muscle. Biochem. Soc. Tr., p. 552.
Carter, N., Jeffery, S., Shiels, A., Edwards, Y., Tipler, T., and Hopkinson, D. A. (1980). Characterization of human carbonic anhydrase III from skeletal muscle. Biochem. Genet. 17837.
Holmes, R. S. (1976). Mammalian carbonic anhydrase isozymes: Evidence for a third locus. J. Exp. Zool. 197289.
Laemli, Y. N. (1979). Clearance of structural proteins during the assembly of the head of bacteriophage T4. Nature 227680.
Moynihan, J. B. (1977). Carbonic anhydrase activity in mammalian skeletal and cardiac muscle. Biochem. J. 168567.
Nørgaard-Pederson, B. (1973). Red cell carbonic anhydrase, Vol. C of Manual of Quantitative Immunoelectrophoresis. Scand. J. Immunol. 2:Suppl. 1.
Register, A. M., Koester, M. K., and Noltmann, E. A. (1978). Discovery of carbonic anhydrase in rabbit skeletal muscle and evidence for its identity with “basic muscle protein.” J. Biol. Chem. 2534143.
Tashian, R. E. (1978). Evolution of the carbonic anhydrases. In Matsubara, H., and Yamonako, T. (eds.), Evolution of Protein Molecules Japan Scientific Societies Press, Tokyo, pp. 343–360.
Taylor, A. W., Essen, B., and Saltin, B. (1974). Myosin ATPase in skeletal muscle of healthy man. Acta Physiol. Scand. 91568–570.
Tipler, T. D., Edwards, Y. H., and Hopkinson, D. A. (1978). Developmental changes in the protein profiles of human cardiac and skeletal muscle. Ann. Hum. Genet. 41409.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jeffery, S., Edwards, Y. & Carter, N. Distribution of CAIII in fetal and adult human tissue. Biochem Genet 18, 843–849 (1980). https://doi.org/10.1007/BF00500117
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00500117