Summary
After i.v. injection in the rat, d-tubocurarine is taken up and concentrated by the liver. A method is developed for the visualisation of d-tubocurarine inside the liver cell by electron microscopy. Glutaraldehyde fixed liver blocks were immersed in an ammonium molybdate solution; d-tubocurarine was precipitated at sites of high concentration by molybdate, to form an insoluble d-tubocurarine-molybdate complex. This precipitate was found predominantly at the surface of lysosome-like particles, but also inside these organelles. In subcellular fractionation experiments, d-tubocurarine was found with a high relative specific “activity” in the lysosomal fraction, lending support to a lysosomal localisation of d-tubocurarine.
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Weitering, J.G., Mulder, G.J., Meijer, D.K.F. et al. On the localisation of d-tubocurarine in rat liver lysosomes in vivo by electron microscopy and subcellular fractionation. Naunyn-Schmiedeberg's Arch. Pharmacol. 289, 251–256 (1975). https://doi.org/10.1007/BF00499979
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DOI: https://doi.org/10.1007/BF00499979