Summary
The activity of penicillin G production was compared in a well defined medium by native vesicles as well as by calcium alginate gel immobilized vesicles isolated from the protoplasts of Penicillium chrysogenum PQ-96. The activity yield of the immobilized vesicles was 44% in comparison with native vesicles. After 60 h of storage at 4° C the native vesicles showed a rapid decrease in penicillin G production. The storage stability of these vesicles was improved after entrapment inside the calcium alginate gel. After 240 h of storage 1 mg of immobilized vesicular protein catalyzed the production of about 140 nmoles of penicillin G in 1 h.
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Kurzątkowski, W., Kuryłowicz, W. & Paszkiewicz, A. Penicillin G production by immobilized fungal vesicles. European J. Appl. Microbiol. Biotechnol. 15, 211–213 (1982). https://doi.org/10.1007/BF00499957
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DOI: https://doi.org/10.1007/BF00499957