Summary
The presence of vitamin-D-dependent calcium-binding protein (CaBP-9K) in tibial growth-plate cartilage was immunohistochemically demonstrated using a specific antibody to rat duodenal CaBP-9K. The protein was found to be mainly localized in the cytoplasm of maturing chondrocytes. In hypertrophic chondrocytes, CaBP-9K concentrations decreased, and the protein was found in the cytoplasmic processes. No CaBP-specific immunoreactivity was seen in the hypertrophic chondrocytes of the lower calcified hypertrophic zone; in contrast, the protein was found in the extracellular lateral edges of longitudinal septa, i.e. where matrix vesicles are preferentially localized and where cartilage mineralization is initiated. These findings suggest that vitamin D has a direct function in this tissue. It also seems likely that CaBP-9K is an indicator of chondrocyte maturation, and that it is involved in the matrix vesicle-associated process of cartilage calcification.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson HC (1969) Vesicles associated with calcification in the matrix of epiphyseal cartilage. J Cell Biol 41:59–72
Arnold B, Kovacs K, Murray TM (1976) Cellular localization of intestinal calcium-binding protein in pig duodenum. Digestion 14:77–84
Cecil RNA, Anderson HC (1978) Freeze-fracture studies of matrix vesicle calcification in epiphyseal growth plate. Metab Bone Dis Rel Res 1:89–95
Celio MR, Norman AW, Heizmann CW (1984) Vitamin D-dependent calcium-binding protein and parvalbumin occur in bones and teeth. Calcif Tissue Int 36:129–130
Delorme AC, Marche P, Garel JM (1979) Vitamin D-dependent calcium-binding protein changes during gestation, prenatal and postnatal development in the rats. J Dev Physiol 1:181–194
Delorme AC, Danan JL, Ripoche MA, Mathieu H (1982) Biochemical characterization of mouse vitamin D-dependent calcium-binding protein. Evidence for its presence in embryonic life. Biochem J 205:49–57
Delorme AC, Cassier P, Geny B, Mathieu H (1983a) Immunocytochemical localization of vitamin D-dependent calcium-binding protein in the yolk sac of the rat. Placenta 4:263–270
Delorme AC, Danan JL, Mathieu H (1983b) Biochemical evidence for the presence of two vitamin D-dependent calcium-binding protein in mouse kidney. J Biol Chem 258:1878–1884
Eneström S, Hed J (1982) Combined immunomorphological examination of epon embedded human kidney biopsies. A comparative study between cryostat, paraffin and consecutive epon-embedded sections for detection and localization of immune deposits. Am J Clin Pathol 77:249–258
Heizmann CW (1984) Parvalbumin, an intracellular calcium-binding protein; distribution, properties and possible roles in mammalian cells. Experentia 40:910–921
Hermanns W, Liebig K, Schulz LC (1981) Postembedding immunohistochemical demonstration of antigen in experimental polyarthritis using plastic embedded whole joints. Histochemistry 73:439–446
Hitchman AJW, Harrison JE (1972) Calcium-binding proteins in the duodenal mucosa of the chick, rat, pig and human. Can J Biochem 50:758–765
Lewinson D, Boskey AL (1984) Calmodulin localization in bone and cartilage. Cell Biol Int Rep 8:11–17
Lewinson D, Silbermann M (1978) Chondrocyte involvement in condylar cartilage calcification utilizing potassium pyroantimonate osmium tetroxide. Metab Bone Dis Rel Res 1:243–250
Marche P, Pradelles P, Gros C, Thomasset M (1977) Radioimmunoassay for a vitamin D-dependent calcium-binding protein in rat duodenal mucosa. Biochem Biophys Res Commun 76:1020–1026
Marche P, Le Guern C, Cassier P (1979a) Immunocytochemical localization of a calcium-binding protein in the rat duodenum. Cell Tissue Res 197:69–77
Marche P, Le Guern C, Cassier P (1979b) Cytoplasmic localization of the calcium-binding protein in rat duodenal absorptive cells. In: Vitamin D, basic research and its clinical application. W de Gruyter, Berlin
Marche P, Cassier P, Mathieu H (1980) Intestinal calcium-binding protein: a protein indicator of enterocyte maturation associated with the terminal web. Cell Tissue Res 212:63–72
Moriuchi S, Yamanouchi T, Hosoya N (1975) Demonstration of two different vitamin D-dependent calcium-binding proteins in rat intestinal mucosa. J Nutr Sci Vitaminol 21:251–259
Rhoten WB, Christakos S (1981) Immunocytochemical localization of vitamin D-dependent calcium-binding protein in mammalian nephron. Endocrinology 109:981–983
Roth J, Brown D, Norman AW, Orci L (1982) Localization of the vitamin D-dependent calcium-binding protein in mammalian kidney. Am J Physiol 243:F243-F252
Schreiner DS, Jande SS (1983) Immunohistochemical localization of low molecular weight water soluble protein-antigens. Immunol Commun 12:W4–5
Schreiner DS, Jande SS, Parkes CO, Lawson DEM, Thomasset M (1983) Immunocytochemical demonstration of two vitamin D-dependent calcium-binding proteins in mammalian kidney. Acta Anat 117:1–14
Streeferk JG (1972) Inhibition of erythrocyte pseudoperoxydase activity by treatment with hydrogen peroxide following methanol. J Histochem Cytochem 20:829–831
Suda S, Takahashi N, Shinki T, Horiuchi N, Yamaguchi A, Yoshiki S, Enomoto S, Suda T (1985) 1α,25-dihydroxyvitamin D3 receptors and their action in embryonic chick chondrocytes. Calcif Tissue Int 37:82–90
Takamiya H, Bodemer W, Vogt A (1978) Masking of protein antigen by modification of amino groups with carbobenzoxychloride (benzyl chloroformate) and demasking by treatment with non specific protease. J Histochem Cytochem 26:914–920
Taylor AN (1983) Vitamin D-dependent calcium-binding proteins: immunocytochemical studies, 1980 to 1983. In: de Bernard B et al. (eds) Calcium-binding proteins. Elsevier, Amsterdam, pp 208–213
Taylor AN (1984) Tooth formation and the 28,000 Dalton vitamin D-dependent calcium-binding protein: an immunocytochemical study. J Histochem Cytochem 32:159–164
Taylor AN, Gleason WA, Landford GL (1984) Renal distribution of the 10,000 dalton vitamin D-dependent calcium-binding protein in neonatal rats. In: Epithelial calcium and phosphate transport: Molecular and cellular aspects. Alan R Liss New York, pp 199–200
Thomasset M, Cuisinier-Gleizes P, Mathieu H (1979) Vitamin D dependence of rat intestinal calcium-binding protein. FEBS Lett 107:91–94
Thomasset M, Parkes CO, Cuisinier-Gleizes P (1982) Rat calcium-binding proteins: distribution, development and vitamin D-dependence. Am J Physiol 243:E483-E488
Thomasset M, Desplan C, Parkes O (1983) Rat vitamin D-dependent calcium-binding proteins. Specificity of mRNAs coding for the 7,500-Mr protein from duodenum and the 28,000-Mr protein from kidney and cerebellum. Eur J Biochem 129:519–524
Underwood JL, DeLuca HF (1984) Vitamin D is not necessary for bone growth and mineralization. Am J Physiol 246:E493-E498
Warembourg M, Tranchant O, Perret C, Desplan C, Thomasset M (in press) In situ detection of cholecalcin (cholecalciferol-induced calcium-binding protein, 9k-Da CaBP) messenger RNA in the rat duodenum. J Histochem Cytochem
Wasserman RH, Taylor AN (1966) Vitamin D-induced calcium-binding protein in chick intestinal mucosa. Science 152:791–793
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Balmain, N., Tisserand-Jochem, E., Thomasset, M. et al. Vitamin-D-dependent calcium-binding protein (CaBP-9K) in rat growth cartilage. Histochemistry 84, 161–168 (1986). https://doi.org/10.1007/BF00499828
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00499828