Abstract
Ribosomal RNA (rRNA) contents were determined in 16 maize genotypes whose individual rRNA gene numbers varied from 5000 to 23,000 per 2C nucleus. Analytical polyacrylamide gel electrophoresis of total RNA showed that no obvious relation existed between rRNA gene number and rRNA content. Only two of nine common inbred lines contained more rRNA than W-23, the inbred with the lowest rRNA gene number. Two of four lines with altered protein content (due to long-term experimental selection) had rRNA contents significantly reduced from those of W-23. A line with an apparent duplication of the nucleolus organizer region of chromosome 6 (called 2-NOR) was expected to possess an elevated quantity of rRNA because it possesses a larger nucleolus; however, we produced a 2-NOR isogenic version and found no difference in rRNA content. The rRNA genes in maize are distributed throughout the NOR-heterochromatin and the NOR-secondary constriction portions of the NOR. The absence of an obvious correlation between rRNA gene number and cellular rRNA content may reflect the presence of a large number of rRNA genes in an inactive state, at least during the stage of growth examined in these experiments.
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Adesnik, M. (1971). In Maramorosch, K., and Koprowski, H. (eds.), Methods in Virology, Vol. V Academic Press, New York, pp. 125–177.
Birnstiel, M. L., Chipchase, M., and Spiers, J. (1971). The ribosomal RNA cistrons. Prog. Nucl. Acid Res. Mol. Biol. 11:351.
Brambl, R., Wenzler, H., and Josephson, M. (1978). Mitochondrial biogenesis during fungal spore germination: Effects of the antilipogenic antibiotic cerulenin upon Botryodiplodia spores. J. Bacteriol. 135:311.
Dudley, J. W. (1974). Seventy Generations of Selection for Oil and Protein in Maize Crop Science Society of America, Madison, Wis., 212 p.
Franz, G., and Kunz, W. (1981). Intervening sequences in ribosomal RNA genes and bobbed phenotype in Drosophila hydei. Nature 292:638.
Givens, J. F., and Phillips, R. L. (1976). The nucleolus organizer region of maize (Zea mays L.). Ribosomal RNA gene distribution and nucleolar interactions. Chromosoma 57:103.
Goldberg, R. B., Bemis, W. P., and Siegel, A. (1972). Nucleic acid hybridization studies within the genus Cucurbita. Genetics 72:253.
Mascia, P. N., Rubenstein, I., Phillips, R. L., Wang, A. S., and Lu, Z. X. (1981). Localization of the 5S rRNA genes and evidence for diversity in the 5S rDNA region of maize. Gene 15:7.
Miller, O. L., Jr. (1981). The nucleolus, chromosomes, and visualization of genetic activity. Cell 91:15s.
Mohan, J., and Ritossa, F. M. (1970). Regulation of ribosomal RNA synthesis and its bearing on the bobbed phenotype in Drosophila melanogaster. Dev. Biol. 22:495.
Perry, R. P. (1981). RNA processing comes of age. Cell 91:28s.
Phillips, R. L. (1978a). In Walden, D.B. (ed.), Genetics and Breeding of Maize Wiley & Sons, New York, pp. 711–741.
Phillips, R. L. (1978b). In Loden, H. D., and Wilkinson, D. (eds.), Proc. Ann. Corn and Sorghum Res. Conf., Vol. 32 Am. Seed Trade Assoc., Washington, D.C., pp. 6–20.
Phillips, R. L., Kleese, R. A., and Wang, S. S. (1971). The nucleolus organizer region of maize (Zea mays L.): Chromosomal site of DNA complementary to ribosomal RNA. Chromosoma 36:79.
Phillips, R. L., Weber, D. F., Kleese, R. A., and Wang, S. S. (1974). The nucleolus organizer region of maize (Zea mays L.): Tests for ribosomal gene compensation or magnification. Genetics 77:285.
Phillips, R. L., Wang, A. S., Rubenstein, I., and Park, W. D. (1979). Hybridization of ribosomal RNA to maize chromosomes. Maydica 24:7.
Phillips, R. L., Wang, A. S., and Kowles, R. V. (1983). In Gustafson, J. P. (ed.), Stadler Genetics Symposium, Vol. 15 Missouri Agricultural Experiment Station, Columbia, pp. 105–118.
Puckett, L. D., and Snyder, L. A. (1974). Quantitative aspects of ribosomal RNA synthesis during ovarian development in two mutants of Drosophila melanogaster. Biochem. Genet. 11:249.
Puckett, L. D., and Snyder, L. A. (1975). Biochemical evidence for postion-effect suppression of ribosomal RNA synthesis in Drosophila melanogaster. Exp. Cell Res. 95:31.
Reeder, R. H., Roan, J. G., and Dunaway, M. (1983). Spacer regulation of Xenopus ribosomal gene transcription: Competition in oocytes. Cell 35:449.
Weinmann, R. (1972). Regulation of ribosomal RNA and 5S RNA synthesis in Drosophila melanogaster. I. Bobbed mutants. Genetics 72:267.
Wimber, D. E., Duffey, P. A., Steffensen, D. M., and Prensky, W. (1974). Localization of the 5S RNA genes in Zea mays by RNA-DNA hybridization in situ. Chromosoma 47:353.
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This research was supported in part by NIH Research Grant GM-19398 to R.B. from the National Institute of General Medical Sciences. This is Paper Number 13,791, Scientific Journal Series, of the Minnesota Agricultural Experiment Station; Saint Paul, Minnesota 55108.
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Buescher, P.J., Phillips, R.L. & Brambl, R. Ribosomal RNA contents of maize genotypes with different ribosomal RNA gene numbers. Biochem Genet 22, 923–930 (1984). https://doi.org/10.1007/BF00499483
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DOI: https://doi.org/10.1007/BF00499483