Abstract
Buoyant density in CsCl, melting temperature, and G + C base content of the DNA from four species of Akodon (Rodentia, Cricetidae) were determined. The buoyant density values of 1.699–1.701 g/cm3 were in accordance with the data reported for other cricetids. No satellite bands were seen in neutral CsCl. The T m values determined in 1 × SSC ranged from 86.2 to 87.0 C, which corresponds to G + C contents of 41.2–43.2%. There was good agreement in DNA base composition of the four species, although values were slightly higher in A. obscurus, suggesting a certain degree of interspecies variability.
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Arrighi, F. E., Mandel, M., Bergendhal, J., and Hsu, T. C. (1970). Buoyant density of DNA of mammals. Biochem. Genet. 4367.
Bianchi, N. O., and Contreras, J. R. (1967). The chromosomes of the field mouse A. azarae (Cricetidae, Rodentia) with special reference to sex chromosomes anomalies. Cytogenetics 6306.
Bianchi, N. O., Reig, O., Molina, O., and Dulout, F. N. (1971). Cytogenetics of the South American rodents (Cricetidae). I. A progress report from Argentinian and Venezuelan forms. Evolution 25724.
Bianchi, N. O., Vidal-Rioja, L., and Bianchi, M. (1973). Constitutive heterochromatin, G bands and Robertsonian rearrangements in the chromosomes of A. molinae. (Rodentia, Cricetidae). Can. J. Genet. Cytol. 15855.
Bianchi, N. O., Vidal-Rioja, L., and Bianchi, M. (1976). Cytogenetics of the South American Akodon rodents (Cricetidae). II. Interspecific homology of G-banding patterns. Cytologia 41139.
Johnson, K. M., Halstead, S. B., and Cohen, S. N. (1967). Hemorrhagic fevers of Southeast Asia and South America: A comparative appraisal. Progr. Med. Virol. 9105.
Kravetz, F. (1977). Ecologia y control de reservorios. Ciencia e Investigación (Asociación Argentina para el progreso de las Ciencias). T.33, No. 7 10:235.
Mandel, M., and Marmur, J. (1968). Use of ultraviolet-absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol. 12B195.
Mandel, M., Schildkraut, C. L., and Marmur, J. (1968). Use of CsCl density analysis for determining the guanine plus cytosine content of DNA. Methods Enzymol. 12B184.
Marmur, J. (1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3208.
Parodi, A., de la Barrera, J. M., and Rugiero, H. R. (1959). Los reservorios del virus Junin. Prensa Med. Argent. 46(9554.
Reig, O., Olivo, N., and Kiblisky, P. (1971). The idiogram of the Venezuelan vole mouse, Akodon urichi venezuelensis Allen (Rodentia, Cricetidae). Cytogenetica 1099.
Schildkraut, C. L., Marmur, J., and Doty, P. (1962). Determination of the base composition of deoxyribonucleic acid (DNA) from its buoyant density in CsCl. J. Mol. Biol. 4430.
Yonenaga, Y. (1972). Chromosomal polymorphism in the rodent Akodon arviculoides ssp (2n=14) resulting from two pericentric inversions. Cytogenetics 11488.
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This study was supported by grants from Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, and Organización de Estados Americanos.
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Vidal-Rioja, L., Semorile, L. & Bianchi, N.O. DNA of Akodon (Rodentia, Cricetidae). I. Biophysical characterization. Biochem Genet 17, 297–303 (1979). https://doi.org/10.1007/BF00498970
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DOI: https://doi.org/10.1007/BF00498970