Summary
Leucocidin from Pseudomonas aeruginosa (strain 158) induced loss of potassium from isolated hepatocytes. The (Na+−K+)-stimulated ATPase activity of isolated rat liver plasma membranes showed dose-dependent activation up to 56%. Electron-spinresonance (ESR) measurements gave no indication of toxin-induced changes in membrane fluidity. On isolated guinea pig heart auricles the toxin produced an increase in frequency from 180/min to about 300/min, with arrhythmia and transitory flutter. On isolated nerve-diaphragm preparations the toxin caused a contracture and a decline in twitch tension, with a loss of potassium into the bathing solution. The action potential of the electrically stimulated N. ischiadicus of rat or frog was not affected when leucocidin was added to the bathing solution up to a concentration of 10 μg/ml.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Antoni, H.: Electrophysiological mechanisms underlying pharmacological models of cardiac fibrillation. Naunyn-Schmiedeberg's Arch. Pharmacol. 269, 177–199 (1971)
Anwer, M. S., Kroker, R., Hegner, D.: Bile acid secretion and synthesis by isolated rat hepatocytes. Biochem. biophys. Res. Commun. 64, 603–609 (1975)
Bülbring, E.: Observations on the isolated phrenic nerve diaphragm of the rat. Brit. J. Pharmacol. 1, 38–61 (1946)
Chen, P. S., Toribara, T. Y., Warner, H.: Microdetermination of phosphorus. Analyt. Chem. 28, 756–759 (1956)
Coleman, R., Michell, R. H., Finean, I. B., Hawthorne, I. N.: A purified plasma membrane fraction isolated from rat liver under isotonic conditions. Biochim. biophys. Acta (Amst.) 135, 573–579 (1967)
Davis, F. A., Jacobson, S.: Altered thermal sensitivity in injured and demyelinated nerve. J. Neurol. Neurosurg. Psychiat. 34, 551–561 (1971)
Emmelot, P., Bos, C. J.: On the participation of neuraminidase sensitive sialic acid in the K+-dependent phosphohydrolysis of p-nitrophenylphosphate by isolated rat-liver plasma membranes. Biochim. biophys. Acta (Amst.) 115, 244–247 (1966)
Emmelot, P., Bos, C. J., Benedetti, E. L., Rümke, Ph.: Studies on plasma membranes. Biochim. biophys. Acta (Amst.) 90, 126–145 (1964)
Franklin, M., Franklin, M. A.: Pseudomonas, ein bakteriologisches Profil. University of New Brunswick, Fed. Kanada, pp. 7–88. Boppard 1973
Frimmer, M., Kroker, R.: The role of extracellular K+-concentration in phalloidin effects on isolated hepatocytes and perfused rat livers. Naunyn-Schmiedeberg's Arch. Pharmacol. 278, 285–292 (1973)
Frimmer, M., Scharmann, W.: Toxicity of a highly purified leucocidin from Pseudomonas aeruginosa in perfused rat livers. Naunyn-Schmiedeberg's Arch. Pharmacol. 288, 123–132 (1975)
Gladstone, G. P., Van Heyningen, W. E.: Staphylococcal leucocidins. Brit. J. exp. Path. 38, 123–137 (1957)
Hegner, D., Schummer, U., Schnepel, G. H.: The interaction of a lytic peptide, melittin with spin-labeled membranes. Biochim. biophys. Acta (Amst.) 291, 15–22 (1973)
Ingebretsen, W. R., Wagle, S. R.: A rapid method for the isolation of large quantities of rat liver parenchymal cells with high anabolic rates. Biochem. biophys. Res. Commun. 47, 403–410 (1972)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurements with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Michell, R. H., Hawthorne, I. N.: The site of diphosphoinositide synthesis in rat liver. Biochem. biophys. Res. Commun. 21, 333–338 (1965)
Robinson, J. D.: Specific modifications of the Na+, K+-dependent adenosine triphosphatase by dimethyl sulfoxide. Ann. N. Y. Acad. Sci. 243, 60–72 (1975)
Scharmann, W.: Leukozidin von Pseudomonas aeruginosa. Habilitationsschrift, Justus-Liebig-Universität Gießen, FB 18 (1975)
Specht, S. C., Robinson, J. D.: Stimulation of the (Na+−K+)-dependent adenosine triphosphatase by amino acids and phosphatidylserine: Chelation of trace metal inhibitors. Arch. Biochem. Biophys. 154, 314–323 (1973)
Stahl, W. L.: Role of phospholipids in the (Na+−K+)-stimulated adenosine triphosphatase system of brain mikrosomes. Arch. Biochem. Biophys. 154, 56–67 (1973)
Wagle, S. R., Ingebretsen, W. R.: Studies on the effect of collagenase and hyaluronidase on glycogen content of isolated rat liver parenchymal cells. Proc. Soc. exp. Biol. (N. Y.) 147, 581–584 (1974)
Weiss, E., Sterz, I., Frimmer, M., Kroker, R.: Electron microscopy of isolated rat hepatocytes before and after treatment with phalloidin. Beitr. Path. 150, 345–356 (1973)
Woodin, A. M.: Purification of the two components of leucocidin from staphylococcus aureus. Biochem. J. 75, 158–165 (1960)
Woodin, A. M., Wieneke, A. A.: Leucocidin, tetraethylammonium ions and the membrane acyl phosphatases in relation to the leucocyte potassium pump. J. gen. Physiol. 56, 16–32 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hegner, D., Petter, A., Kroker, R. et al. Leucocidin from Pseudomonas aeruginosa and membrane functions. Naunyn-Schmiedeberg's Arch. Pharmacol. 293, 49–55 (1976). https://doi.org/10.1007/BF00498870
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00498870