Summary
The renal effects of dopamine were studied using clearance and micropuncture techniques in rats. Intravenous infusion of dopamine (4.7 · 10−6 mol · kg−1 · h−1) increased glomerular filtration rate and renal blood flow. Renal blood flow was measured by an electromagnetic flowmeter. The increase in filtered fluid and sodium was nearly completely matched by increased tubular reabsorption. Thus, only a small rise in urine flow and in urinary sodium excretion was observed.
The micropuncture experiments using the split oil droplet method of Gertz demonstrated a stimulation of the transepithelial fluid transfer after injection of dopamine (10−4M) into the proximal tubular lumen. This effect was abolished by simultaneous injection of propranolol (10−3 M) which, by its own, did not affect transtubular volume fluxes. It is concluded that dopamine, by stimulation of β-adrenoceptors, may increase reabsorptive capacity of the proximal tubular epithelium independent of changes in renal hemodynamics.
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Black, W. L., Rolett, E. L.: Cardiovascular adrenergic activity of dopamine in the dog. Amer. Heart J. 75, 233–239 (1968)
Davis, B. B., Walter, M. J., Murdaugh, H. V.: The mechamism of the increase in sodium excretion following dopamine infusion. Proc. Soc. exp. Biol. (N.Y.) 129, 210–213 (1968)
Führ, J., Kaczarczyk, J., Krüttgen, C. D.: Eine einfache Methode zur Inulinbestimmung für Nieren-Clearance-Untersuchung bei Stoffwechselgesunden und Diabetikern. Klin. Wschr. 33, 729–730 (1955)
Fülgraff, G., Heidenreich, O.: Mikropunktionsuntersuchungen über die Wirkung von Calciumionen auf die Resorptionskapazität und auf die prozentuale Resorption im proximalen Konvolut von Ratten. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 258, 440–451 (1967)
Fülgraff, G., Heidenreich, O., Heintze, K., Osswald, H.: Die Wirkung von α-und β-Sympathomimetica und Sympatholytica auf die renale Exkretion und Resorption von Flüssigkeit und Elektrolyten in Ausscheidungs- und Mikropunktionsversuchen an Ratten. Naunyn-schmiedebergs Arch. Pharmak. exp. Path. 262, 295–308 (1969)
Gertz, K. H.: Transtubuläre Natriumchloridflüsse und Permeabilität für Nichtelektrolyte im proximalen und distalen Konvolut der Rattenniere. Pflügers Arch. ges. Physiol. 276, 336–356 (1963)
Gertz, K. H.: Die Anpassung der transtubulären Resorption an die glomeruläre Filtrationsrate. In: Normale und path. Funktion des Nierentubulus (K. J. Ullrich u. K. Hierholzer, Hrsg.). Bern-Stuttgart: H. Huber 1965
Goldberg, L. I.: Cardiovascular and renal actions of dopamine: potential clinical applications. Pharmacol. Rev. 24, 1–29 (1972)
Györy, A. Z.: Reexamination of the split droplet method as applied to kidney tubules. Pflügers Arch. 324, 328–343 (1971)
Hierholzer, K., Wiederholt, M., Stolte, H.: Hemmung der Natriumresorption im proximalen und distalen Konvolut adrenalektomierter Ratten. Pflügers Arch. ges. Physiol. 291, 43–62 (1966)
McDonald, R. H., Goldberg, L. I.: Analysis of the cardiovascular effects of dopamine in the dog. J. Pharmacol. exp. Ther. 140, 60–66 (1963)
McNay, I. L., Goldberg, L. I.: Comparison of the effect of dopamine, isoproterenol, norepinephrine and bradykinin on canine renal and femoral blood flow. J. Pharmacol. exp. Ther. 151, 23–31 (1966)
Rector, F. C., sellman, J. C., Martinez-Maldonado, M., Seldin, D. W.: The mechanism of suppression of proximal tubular reabsorption by saline infusions. J. clin. Invest. 46, 47–58 (1967)
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Greven, J., Klein, H. Effects of dopamine on whole kidney function and proximal transtubular volume fluxes in the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 296, 289–292 (1977). https://doi.org/10.1007/BF00498695
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DOI: https://doi.org/10.1007/BF00498695