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Excretion of metabolites by hydrogen bacteria

III. D(−)-3-hydroxybutanoate

  • Industrial Microbiology
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Summary

D(−)-3-hydroxybutanoic acid was produced by a double mutant of Alcaligenes eutrophus, unable to synthesize poly-3-hydroxybutanoic acid and to utilize 3-hydroxybutanoate as a substrate. About 3.4 g of 3-hydroxybutanoate/l were produced under optimum conditions at pH 7.0 to 7.3, at a temperature of 30°C after exhaustion of ammonia and at restricted aeration rates allowing 10–12% of the maximum respiratory rate of the cells to occur. D(−)-3-hydroxybutanoic acid was identified by means of gas and ion exchange chromatography, IR-spectrometry and specific rotation.

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Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Göttingen, Grisebachstr. 8, D-3400, Göttingen, Federal Republic of Germany

    D. Vollbrecht & H. G. Schlegel

  2. der Gesellschaft für Strahlen- und Umweltforschung mbH, München, Grisebachstr. 8, D-3400, Göttingen, Federal Republic of Germany

    D. Vollbrecht & H. G. Schlegel

Authors
  1. D. Vollbrecht
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  2. H. G. Schlegel
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Vollbrecht, D., Schlegel, H.G. Excretion of metabolites by hydrogen bacteria. European J. Appl. Microbiol. Biotechnol. 7, 259–266 (1979). https://doi.org/10.1007/BF00498020

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  • DOI: https://doi.org/10.1007/BF00498020

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