Abstract
Antiserum was raised in sheep against canine C-reactive protein (CRP) and antibody, which was not specific for CRP, was removed by absorption with normal canine serum protein linked to agarose beads. The antiserum was used to develop an immunoturbidimetric assay for canine CRP on a MIRA (Roche Diagnostics) automated clinical biochemical analyser and assessed for routine analysis of CRP in canine serum samples. The assay gave standard curves with each standard having a coefficient of variance (CV) between 4.8 and 11%, interassay CVs below 11% and intra-assay CVs of less than 5%. Parallel dilution curves were obtained with purified CRP diluted in buffer and with endogenous CRP in serum diluted with buffer or with a serum with a negligible CRP content. The immunoturbidimetric assay results correlated with the results obtained using an ELISA method, r=0.88. The immunoturbidimetric assay of canine CRP proved to be suitable for the routine analysis of canine CRP.
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References
Brun, E., Lingaas, F. and Larsen, H.J., 1989. Turbidimetric measurement of immunoglobulin G in serum using an automatic centrifugal analyser. Research in Veterinary Science, 46, 168–171
Caspi, D., Baltz, M.L., Snel, F., Gruys, E., Niv, D., Batt, R.M., Munn, E.A., Buttress, N. and Pepys, M.B., 1984. Isolation and characterization of C-reactive protein from dog. Immunology, 53, 307–313.
Caspi, D., Snel, F.W., Batt, R.M., Bennett, D., Rutteman, G.R., Hartman, E.G., Baltz, M.L., Gruys, E. and Pepys, M.B., 1987. C-Reactive protein in dogs. American Journal of Veterinary Research, 48, 919–921
Conner, J.G., Eckersall, P.D., Ferguson, J. and Douglas, T.A., 1988. Acute phase response in the dog following surgical trauma. Research in Veterinary Science, 45, 107–110
Eckersall, P.D. and Conner, J.G., 1988. Bovine and canine acute phase proteins. Veterinary Research Communications, 12, 169–178
Eckersall, P.D. and Conner, J.G., 1990. Plasma haptoglobin in cattle (Bos taurus) as polymers in association with albumin. Comparative Biochemistry and Physiology, 96B, 309–314
Eckersall, P.D., Conner, J.G. and Parton, H., 1989. An enzyme-linked immunosorbent assay for canine C-reactive protein. Veterinary Record, 124, 490–491
Kent, J.E. and Blackmore, D.J., 1985a. Measurement of IgG in equine blood by immunoturbidimetry and latex agglutination. Equine Veterinary Journal, 17, 125–129
Kent, J.E. and Blackmore, D.J., 1985b. Turbidimetric measurement of IgG(T) in the serum of healthy thoroughbreds and ponies. Equine Veterinary Journal, 17, 119–124
Kushner, I. and Mackiewicz, A., 1987. Acute phase proteins as disease markers. Disease Markers, 5, 1–11
Pepys, M.B. and Baltz, M.L., 1983. Acute phase proteins with special reference to C-reactive protein and related proteins (Pentraxins) and serum amyloid A protein. Advances in Immunology, 34, 141–212
Ritchie, R.F., 1967. A simple, direct and sensitive technique for the measurement of specific protein in dilute solution. Journal of Laboratory Clinical Medicine, 70, 512–517
Sehgal, P.B., Greininger, G. and Tosato, G., 1989. Regulation of the acute phase responses: interleukin-6. Annals of the New York Academy of Sciences, Volume 557
Winkles, J., Lunec, J. and Deverill, I., 1987. Enhanced-latex-agglutination assay for C-reactive protein in serum, with use of a centrifugal analyser. Clinical Chemistry, 33, 685–689
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Eckersall, P.D., Conner, J.G. & Harvie, J. An immunoturbidimetric assay for canine C-reactive protein. Vet Res Commun 15, 17–24 (1991). https://doi.org/10.1007/BF00497786
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DOI: https://doi.org/10.1007/BF00497786