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Immunohistochemical localisation of arginase in human liver using monoclonal antibodies against human liver arginase

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Summary

Monoclonal antibodies against human liver arginase were raised in order to determine the exact distribution of arginase in human liver using a modified indirect unlabelled immunoperoxidase method. In normal human liver specific immunohistochemical staining was found in the cytoplasm of hepatocytes. Portal components (bile ducts and veins) and fibrous tissue were non-reactive, while erythrocytes were slightly positive. The specificity of the immunological reaction was confirmed by control tests. Spectrophotometry was used to quantitate the immunohistochemical reaction product, and the results indicated that arginase is homogeneously distributed in the liver lobule.

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Authors and Affiliations

  1. Department of Haematology, Oncology and Immunology, Robert-Bosch-Krankenhaus, Auerbachstrasse 110, D-7000, Stuttgart 50, Federal Republic of Germany

    H. Multhaupt & K. Schumacher

  2. Department of Clinical and Experimental Pathology, Robert-Bosch-Krankenhaus, Auerbachstrasse 110, D-7000, Stuttgart 50, Federal Republic of Germany

    P. Fritz

Authors
  1. H. Multhaupt
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  2. P. Fritz
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  3. K. Schumacher
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Present address: Biologisches Institut der Universität Stuttgart, Ulmerstrasse 227, D-7000 Stuttgart 60, Federal Republic of Germany

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Multhaupt, H., Fritz, P. & Schumacher, K. Immunohistochemical localisation of arginase in human liver using monoclonal antibodies against human liver arginase. Histochemistry 87, 465–470 (1987). https://doi.org/10.1007/BF00496818

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  • DOI: https://doi.org/10.1007/BF00496818

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