Summary
Calcium was not found in secretory ameloblasts and stratum intermedium cells when treated with OsO4-pyroantimonate or when surfaces prepared by fracturing fresh, rapidly frozen, developing molar tooth germs were subject to electron probe X-ray analysis.
Pyroantimonate reaction product, considered to be calcium, was found in mitochondria of enamel organ cells which were first placed in a bath containing calcium and potassium. The plasma membrane was disrupted in cells which showed mitochondrial localization of reaction product.
The results provide no data which indicates that enamel organ cells have a direct, active role in the movement of calcium into the enamel. Rather, it is suggested that the secretory enamel organ might serve as a selective barrier in regulating the initial mineralization of enamel.
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References
Arsenis, C.: Role of mitochondria in calcification. Biochem. Biophys. Res. Comm. 46, 1928–1935 (1972)
Baker, P.F.: The regulation of intracellular calcium. In: Symposium XXX, Calcium in Biological Systems, Symposia of the Society for Experimental Biology, pp. 67–88. Cambridge, England: Cambridge University Press 1976
Bawdon, W., Wennberg, A.: In vitro study of cellular influence on Ca uptake in developing rat enamel. J. Dent. Res. 56, 313–319 (1977)
Borle, A.B., Anderson, J.H.: A cybernetic view of cell calcium metabolism. In: Symposium XXX, Calcium in Biological Systems. Symposia of the Society for Experimental Biology, pp. 141–160. Cambridge. England: Cambridge University Press 1976
Boyde, A.: Real-time stereo TV speed scanning electron microscopy. Beitr. elektronenmikroskop. Direktabb. Oberfl. 7, 221–230 (1974)
Boyde, A., Reith, E.J.: Qualitative electron probe analysis of secretory ameloblasts and odontoblasts in the rat incisor. Histochemistry 50, 347–354 (1977)
Brighton, C.T., Hunt, R.M.: Mitochondrial calcium and its role in calcification. Clin. Orthop. 100, 406–416 (1974)
Carafoli, E., Crompton, M.: Calcium ions and mitochondria. In: Symposium XXX, Calcification in Biological Systems. Symposia of the Society for Experimental Biology, pp. 89–115. Cambridge, England: Cambridge University Press 1976
Clark, M.A., Ackerman, G.A.: A histochemical evaluation of the pyroantimonate-osmium reaction. J. Histochem. Cytochem. 19, 727–737 (1971)
Cohn, D.V., Bawdon, E.G.: The effect of parathyroid hormone in vivo on the accumulation of calcium and phosphate by kidney and on kidney mitochondrial function. J. Biol. Chem. 242, 1253–1258 (1967).
Debbas, G., Hoffman, L., Landon, E.J., Hurwitz, J.: Electron microscopic localization of calcium in vascular smooth muscle. Anat. Rec. 182, 447–472 (1975)
Irving, T.: Theories of mineralization of bone. Clin. Orthop. 97, 225–236 (1973)
Kashiwa, H.K., Sigman, M.D.: Calcium localized in odontogenic cells of rat mandibular teeth by the glyoxal-bis-(2-hydroxyanil) method. J. dent. Res. 45, 1796–1799 (1965)
Landis, W.J., Hauschka, T., Rogerson, A., Glimcher, M.J.: Electron microscopic observations of bone tissue prepared by ultra cryomicrotomy. J. ultrastruct. Res. 59, 185–206 (1977)
Lehninger, A.C.: Mitochondria and calcium ion transport. Biochem. J. 119, 129–138 (1970)
Martin, J.H., Matthews, J.L.: Mitochondrial granules in chondrocytes. Calcif. Tissue Res. 3, 184–193 (1969)
Martin, J.H., Matthews, J.L.: Mitochondrial granules in chondrocytes, osteoblasts and osteocytes. Clin. Orthop. 68, 273–278 (1970)
Munhoz, C.O.G., Leblond, C.P.: Deposition of calcium phosphate into dentine and enamel as shown by radioutography of sections of incisor teeth following injection of 45Ca into rats. Calcif. Tissue Res. 15, 221–235 (1974)
Nagai, N., Frank, R.M.: Transfert du 45Ca par autoradiographie en microscopie électronique au cours de l'amélogenèse. Calcif. Tissue Res. 19, 211–221 (1975)
Peachey, L.D.: Electron microscopic observations on the accumulation of divalent cations in intramitochondrial granules. J. Cell. Biol. 20, 95–108 (1964)
Reith, E.J., Cotty, V.F.: Autoradiographic studies on calcification of enamel. Arch. Oral Biol. 7, 365–372 (1962)
Sayegh, F.S., Porter, K., Sun, G., Sellers, G.: Ca45 uptake by odontoblasts mitochondria. J. Dent. Res. 55, B114 (1976) (abstract)
Sayegh, F.S., Solomon, C., Davis, W.: Ultrastructure of intracellular mineralization in deer's antler. Clin. Orthop. 99, 267–284 (1974)
Shapiro, I., Greenspan, J.S.: Are mitochondria directly involved in biological mineralization? Calcif. Tissue Res. 3, 100–102 (1967)
Shapiro, I.M., Lee, H.: Calcium accumulation by chondrocyte mitochondria. Clin. Orthop. 106, 323–339 (1975)
Spicer, S.S., Hardin, J.H., Greene, W.B.: Nuclear precipitates in pyroantimonate-osmiumtetroxide fixed tissues. J. Cell. Biol. 39, 216–221 (1968)
Williams, R.J.P.: Calcium chemistry and its relation to biological function. In: Symposium XXX, Calcium in Biological Systems. Symposia of the Society for Experimental Biology, pp. 1–17. Cambridge, England: Cambridge University Press 1976
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Reith, E.J., Boyde, A. Histochemical and electron probe analysis of secretory ameloblasts of developing rat molar teeth. Histochemistry 55, 17–26 (1978). https://doi.org/10.1007/BF00496690
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DOI: https://doi.org/10.1007/BF00496690