Summary
We used the simple method of direct cytofluorescence to detect the presence of the aminoglycoside, streptomycin, in the inner ear after its systemic administration. In the cochlea, fluorescence was observed in the organ of Corti, the spiral ganglion, the nerve fibres, the vascular stria and Reissner's membrane; in the vestibulum, fluorescence was seen in the crista ampullaris and the planum semilunatum. The localization of the drug was related to the distribution of its specific receptor, triphosphoinositide (TPI); therefore, it is reasonable to assume that aminoglycosides exert their toxic effects by binding to TPI.
Similar content being viewed by others
References
Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Harada Y (1983) Reissner's membrane. In: Harada Y (ed) Atlas of the ear by scanning electron microscopy. MTP Press, Lancaster, pp 165–169
Iurato S (1967) Planum semilunatum and dark cells. In: Iurato S (ed) Submicroscopic structure of the inner ear. Pergamon Press, Oxford, pp 219–225
Kimura S (1969) Distribution, structure and function of dark cells in the vestibular labyrinth. Ann Otol Rhinol Laryngol 78:542–561
Mees K (1983) Ultrastructural localization of K+-dependent, ouabain-sensitive NPPase (Na+−K+-ATPase) in the guinea pig inner ear. Acta Otolaryngol 95:277–289
Müsebeck K, Schätzler W (1964) Das Verhalten der suren Mucopolysaccharide der Meerschweinchenschnecke nach Vergiftung mit Dihydrostreptomycin und einem Tetracyclin-Derivat. Arch Ohr Nasen Kehlkopf Heilk 181:530–536
Orsulakova A, Stockhorst E, Schacht J (1976) Effect of neomycin on phophoinositide labelling and calcium binding in guinea pig inner ear tissue in vivo and in vitro. J Neurochem 26:285–290
Saito H (1967) Biochemical and histochemical studies on acid mucopolysaccarides in the cochlea of guinea pigs. Jpn J Otolaryngol 70:662–690
Sande MA, Mandell GL (1980) The Aminoglycosides. In: Goodman Gilman A, Goodman LS, Gilman A (eds) The pharmacological bases of therapeutics. McMillan, New York Toronto London, pp 1245–1263
Schacht J (1974) Interaction of neomycin with phosphoinositide metabolism in guinea pig inner ear and brain tissue. Ann Otol 83:813–818
Schacht J (1979) Isolation of an aminoglycoside receptor from guinea pig inner ear and kidney. Arch Otorhinolaryngol 224:129–134
Schacht J (1982) Adenylate cyclase and cochlear fluid balance. Am J Otolaryngol 3:328–331
Spoendlin H (1967) Vacular Stria. In: Iurato S (ed) Submicroscopic structure of the inner ear. Pergamon Press, Oxford, pp 131–149
Tachibana M, Saito H, Machino M (1973) Sulfated acid mucopolysaccharides in the tectorial membrane. Acta Otolaryngol 76:37–46
Tachibana M, Saito H, Yamamichi I, Morioka H (1978) A possible involvement of acidic glycosaminoglycans in kanamycin ototoxicity. Acta Otolaryngol 86:15–21
Tachibana M, Morioka H, Machino M, Oshima W, Mizukoshi F, Mizukoshi O, Yoshioka T (1984) Localization of triphosphoinositide in the cochlea. An electronmicroscopic immunocytochemical study. Histochemistry 81:157–160
Tachibana M, Morioka M, Machino M, Mizukoshi F, Mizukoshi O, Yoshioka T (1985a) Immunocytochemical detection of triphosphoinositide in vestibular hair cells. Histochemistry 82:197–199
Tachibana M, Morioka H, Machino M, Mizukoshi O (1985b) Binding sites of an aminoglycoside in the cochlea examined by immunocytochemistry. Histochemistry 83:237–240
Von Ilberg C, Spoendlin H, Arnold W (1971) Autoradiographical distribution of locally applied dihydrostreptomycin in the inner ear. Acta Otolaryngol 71:159–165
Weiner ND, Schacht J (1981) Biochemical model of aminoglycoside-induced hearing loss. In: Lerner SA, Matz GJ, Hawkins JE Jr (eds) Aminoglycoside ototoxicity. Little Brown, Boston, pp 197–214
Zajic G, Anniko M, Schacht J (1983) Cellular localization of adenylcyclase in the mature inner ear of the mouse. Hearing Res 10:249–261
Author information
Authors and Affiliations
Additional information
Supported by grants of Ministero della Pubblica Istruzione, Italy
Rights and permissions
About this article
Cite this article
Bareggi, R., Narducci, P., Grill, V. et al. Localization of an aminoglycoside (streptomycin) in the inner ear after its systemic administration. Histochemistry 84, 237–240 (1986). https://doi.org/10.1007/BF00495788
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00495788