Summary
An anti-thymostimulin (TS) serum was tested on calf thymus to study the localization of the hormonal factor. The immunoperoxidase method was applied to tissue fixed in Bouin's fluid and embedded in paraffin, or to tissue fixed in paraformaldehyde and embedded in Epon for semi-thin sections. Immuno-reactivity was shown, with DAB-Fluka, in reticulo-epithelial cells in the medulla, and between the cortex and the medulla, while with DAB-Sigma reactivity was found in the cortex as well. The external cells of Hassall's corpuscles were also reactive. Myoid cells were not reactive. In semi-thin sections a weak reactivity was noted at the periphery of a few lymphocytes. Comparison with the localization of other thymic factors, and the possibility of a functional cycle of the epithelial cells synthesizing one or more factors are discussed.
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Aita M, Amantea A, Minella AB, Cocchia D (1979a) A thymic factor localization in calf thymus. 5th Europ Anat Congr, Prague
Aita M, Ammirati P, Falchetti R, Pezzella M (1981) Immunological and immunohistochemical properties of an anti-thymostimulin (TP. 1) serum. Cell Mol Biol 27:369–375
Aita M, Cocchia D, Amantea A, Minella AB, Ammirati P, Pezzella F (1979b) Localizzazione immunoistochimica di un fattore ormonale timico, Basic Appl Histochem 23 [Suppl]:1
Aita M, Mastrandrea F (1980) Production of a soluble factor in thymus culture: relationship to TP. 1. In: Aiuti F, Wigzell H (eds) Thymus, Thymic hormones and T lymphocytes. Vol 38. Proc Serono Symposia. Academic Press, London, pp 237–243
Aita M, Minella AB, Amantea A, Mastrandrea F, Ammirati P (1979c) Localisation immunohistochimique d'un facteur thymique au niveau du thymus de veau. Bull Assoc Anat Paris 63:99
Audhya T, Schlesinger DH, Goldstein G (1981) Complete amino acid sequences of bovine Thymopoietins I, II, and III: closely homologous polypeptides. Biochemistry 20:6195–6200
Auger C, Monier JC, Dardenne M, Pléau JM, Bach JF (1982) Identifcation of FTS (facteur thymique sérique) on thymus ultrathin sections using monoclonal antibodies. Immunol Lett 5:213–216
Bach JF (1978) Introduction: Thymic hormones. In: Werner GH, Floch A (eds) The pharmacology of immunoregulation. Academic Press, London, pp 165–172
Bach JF, Bach MA, Charreire J, Dardenne M, Fournier C, Papiernik M, Pleau JM (1974) The circulating thymic factor (TF), biochemistry, physiology, biological activities and clinical applications, a summary. In: van Bekkum DW (ed) Biological activity of thymic hormones. Kooyker, Rotterdam, pp 145–158
Bach JF, Dardenne M, Pléau JM (1977) Biochemical characterisation of a serum thymic factor. Nature 266:55–57
Bach JF, Dardenne M, Pléau JM, Rosa J (1976) Caracterisation biochimique du facteur thymique circulant. CR Acad Sci Paris, Ser D, 283:1605–1607
Blau JN (1967) Antigen and antibody localization in Hassall's corpuscles. Nature 215:1073–1075
Brelinska R, Warchol JB (1982) Receptors for thymosin fraction V on rat thymic lymphocytes. Experientia 38:1317–1318
Curran RC, Gregory J (1980) Effects of fixation and processing on immunohistochemical demonstration of immunoglobulin in paraffin sections on tonsil and bone marrow. J Clin Pathol 33:1047–1057
Dardenne M, Bach JF (1976) Ontogeny of thymus factor secretion. Ann Immunol 127c:963–966
Dardenne M, Papiernik M, Bach JF, Stutman O (1973) Studies on thymus products. III Epithelial origin of the serum thymic factor. Immunology 27:299–304
Deschaux P, Doublet A, Fontanges R (1982) Etude comparative du mode d'action intracellulaire de la thymosine α1 et du facteur thymique sérique. CR Acad Sci Paris Ser III 294:207–210
Falchetti R, Bergesi G, Eshkol A, Cafiero C, Adorini L, Caprino L (1977) Pharmacological and biological properties of a calf thymus extract (TP. 1). Drugs Exp Clin Res 3:39–47
Falchetti R, Cafiero C, Caprino L (1982a) Impaired T-cell functions in aged guinea-pigs restored by thymostimulin (TS). Int J Immunopharmacol 4:181–186
Falchetti R, Cafiero C, Diorio U, Caprino L (1982b) Thymostimulin (TS) effects on immunological responses of cyclophophamide treated newborn guinea pigs. Int J Immunopharmacol 4:300
Falchetti R, Caprino L, Cafiero C (1981) Enhancement of the immunocompetence in neonatal guinea pigs by a thymic factor (TS). Thymus 2:215–224
Goldstein AL, Low TLK, Thurman GB, Zazt MM, Hall N, Chen J, Hu SK, Naylor PB, McClure JE (1981) Current status of thymosin and other hormones of the thymus gland. In: Greep RO (ed) Recent progres in hormone research, Vol 37. Academic Press, London New York, pp 369–413
Goldstein AL, Thurman GB, Cohen GH, Hooper JA (1974) Thymosin: chemistry, biology and clinical applications. In: van Bekkum DW (ed) Biological activity of thymic hormones. Kookyer, Rotterdam, pp 173–197
Goldstein G (1975) The isolation of thymopoietin (thymin). Ann NY Acad Sci 249:177–185
Goldstein G, Scheid MP, Boyse EA, Schlesinger DH, van Wauwe J (1979) A synthetic pentapeptide with biological activity characteristic of the thymic hormone thymopoietin. Science 204:1309–1310
Hirokawa K, McClure JE, Goldstein AL (1982) Age-related changes in localization of thymosin in the human thymus. Thymus 4:19–29
Jambon B, Montagne P, Bene MC, Brayer MP, Faure G, Duheille J (1981) Immunohistologic localization of “facteur thymique sérique” (FTS) in human thymic epithelium. J Immunol 127:2055–2059
Kater L, Oosterom, R, McClure J, Goldstein AL (1979) Presence of thymosin-like factors in human thymic epithelium conditioned medium. Int J Immunopharmacol 1:1–12
Kato K, Ikeyama S, Takaoki M, Shino A, Takeuchi M, Kakinuma A (1981) Epithelial cell components immunoreact with antiserum thymic factor (FTS) antibodies: possible association with intermediate-sized filaments. Cell 24:885–895
Low TLK, Goldstein AL (1979) The chemistry and biology of thymosin. II Amino acid sequence analysis of thymosin α1 and polypeptide β1. J Biol Chem 254:987–995
Low TLK, Thurman GB, McAdoo M, McClure J, Rossio JL, Naylor PH, Goldstein AL (1979a) The chemistry and biology of thymosin. I Isolation, characterization and biological activities of thymosin α1 and polypeptide β1 from calf thymus. J Biol Chem 254:981–986
Low TLK, Thurman GB, Chincarini C, McClure JE, Marshall GD, Hu S-K, Goldstein AL (1979b) Current status of thymosin research: evidence for the existence of a family of thymic factors that control T-cell maturation. Ann NY Acad Sci 332:33–48
Low TLK, Hu S-K, Goldstein AL (1981) Complete amino acid sequence of bovine thymosin β4: a thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations. Proc Natl Acad Sci USA 78:1162–1166
Mandi B, Glant T (1973) Thymosin-producing cells of the thymus. Nature (New Biol) 246:25
Mandi B, Holub M, Rossmann P, Csaba B, Glant T, Olveti E (1978) Detection of thymosin 5 in calf and mouse thymus and in nude mouse dysgenetic thymus. Folia Biol (Praha) 25:49–55
McClure JE, Lameris N, Wara DW, Goldstein AL (1982) Immunochemical studies on thymosin: radioimmunoassay of thymosin α1. J Immunol 128:368–375
Miller JFAP (1961) Immunological function of the thymus. Lancet 2:748–749
Monier JC, Dardenne M, Pléau JM, Schmitt D, Deschaux P, Bach JF (1980) Characterization of facteur thymique sérique (FTS) in the thymus. I Fixation of anti-FTS antibodies on thymic reticulo-epithelial cells. Clin Exp Immunol 42:470–476
Osoba D, Miller JFAP (1963) Evidence for a humoral thymus factor responsible for the maturation of immunological faculty. Nature 199:653–654
Rotter V (1974) The effect of THF on the reactivity of lymphoid cells to PHA and Con A. In: van Bekkun DW (ed) Biological activity of thymic hormones. Kooyker, Rotterdam, pp 227–228
Rotter V, Trainin N (1979) Role of thymic hormone (THF) and of a thymic plasma recirculating factor (TPRF) in the modulation of human lymphocyte response to PHA and Con A. J Immunol 122:414–420
Savino W, Dardenne M, Papiernik M, Bach JF (1982) Thymic hormone-containing cells. Characterization and localization of serum thymic factor in young mouse thymus studied by monoclonal antibodies. J Exp Med 156:628–633
Schlesinger DH, Goldstein G (1975) The amino acid sequence of thymopoietin II. Cell 5:361–365
Schlesinger DH, Goldstein G, Scheid MP, Boyse EA (1975) Chemical synthesis of a peptide fragment of thymopoietin II that induces selective T cell differentiation. Cell 5:367–370
Schmitt D, Monier JC, Dardenne M Pléau JM, Bach JF (1982) Location of FTS (facteur thymique sérique) in the thymus of normal and auto-immune mice. Thymus 4:221–231
Schmitt D, Monier JC, Dardenne M, Pléau JM, Deschaux P, Bach JF (1980) Cytoplasmic localization of FTS (facteur thymique sérique) in thymic epithelial cells. An immunoelectronmicroscopical study. Thymus 2:177–186
Schulof RS, Goldstein AL (1977) Thymosin and the endocrine thymus. Adv Intern Med 22:121–143
Soppi E, Eskola J, Goldstein AL, Ruuskanen O (1979) Effect of thymosin on the mitogenic responses of guinea pig thymocytes with different maturation stages. Dev Comp Immunol 3:489–498
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–335
Streefkerk JG (1972) Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol. Histochem Cytochem 20:829–831
Teodorczyk JA, Potoworowski EF, Sviculis A (1975) Cellular localisation and antigenic species specificity of thymic factors. Nature 258:617–619
Touraine JL, Touraine F, Incefy GS, Goldstein AL, Good RA (1974) Thymic factors and human T lymphocyte differentiation. In: van Bekkum DW (ed) Biological activity of thymic hormones. Kooyker, Rotterdam, pp 31–35
Trainin N, Small M, Zipori D, Umiel T, Kook AI, Rotter V (1974) Characteristics of THF, a thymic hormone. In: van Bekkum DW (ed) The biological activity of thymic hormones. Kooyker, Rotterdam, pp 117–143
Twomey JJ, Goldstein G, Lewis VM, Bealmear PM, Good RA (1977) Bioassay determinations of thymopoietin and thymic hormone levels in human plasm. Proc Natl Acad Sci USA 74:2541–2545
Van Bekkum DW (ed) (1975) Biological activity of thymic hormones. Kooyker, Rotterdam
Van den Tweel JG, Taylor CR, McClure J, Goldstein AL (1979) Detection of thymosin in thymic epithelial cells by an immunoperoxidase method. Adv Exp Med Biol 114:511–515
White A, Goldstein AL (1975) The endocrine role of the thymus and its hormone, thymosin in the regulation of the growth and maturation of host immunological competence. Adv Metab Dis 8:359–374
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Aita, M., Cocchia, D., Minella, A.B. et al. Identification of thymostimulin secreting cells in calf thymus by immunoperoxidase method. Histochemistry 80, 207–211 (1984). https://doi.org/10.1007/BF00495767
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DOI: https://doi.org/10.1007/BF00495767