Summary
Sorex belongs to the Insectivora and has a pigmented tooth enamel due to iron. The pigmented enamel (PE) has a mean Ca/P weight ratio, analyzed by quantitative electronprobe X-ray microanalysis, of about 1.9 (mean molar Ca/P ratio 1.46), and the unpigmented enamel (UE) a Ca/P weight ratio of about 2.0 (mean molar Ca/P ratio 1.59). The PE has a higher iron content (with a value of about 8%) than the UE, as shown by microanalysis of ultrathin sections. Laser microprobe mass analysis (LAMMA) has shown that the carbonate content in the UE is higher than in the PE. In the LAMMA spectrum of the negatively charged ions the carbonate lines could be compared directly with those of negatively charged iron ions. The pigmentation is associated with a low Ca/P ratio but may transfer mechanical strength and acid resistance strength to the PE.
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References
Bibra E (1844) Chemische Untersuchungen über die Knochen und Zähne des Menschen und der Wirbeltiere. Schweinfurt (cited by Pindborg et al. 1946)
Boer HH, Witteveen J (1980) Ultrastructural localization of carbonic anhydrase in tissues involved in shell formation and ionic regulation in the pond snail Lymnaea stagnalis. Cell Tissue Res 209:383–390
Boyde A, Switsur VR, Fearnhead RW (1961) Application of the scanning electron-probe X-ray microanalyser to dental tissues. J Ultrastruct Res 5:201–207
Cuvier MF (1825) Les dents des mammiferes. Strasbourg and Paris (cited by Pindborg et al, 1946)
Dötsch CV, Koenigswald WV (1978) Zur Rotfärbung von Soricidenzähnen. Z Säugetierkunde 43:65–70
Gay CV, Faleski EJ, Schraer H, Schraer R (1974) Localization of carbonic anhydrase in avian gastric mucosa, shell gland and bone by immunohistochemistry. J Histochem Cytochem 22:819–825
Halse A (1972) An electron microprobe investigation of the distribution of iron in rat incisor enamel. Scand J Dent Res 80:26–39
Halse A (1974a) Elemental composition of the superficial layer of rat incisor enamel. Calcif Tissue Res 16:139–144
Halse A (1974b) Electron microprobe analysis of iron content of incisor enamel in some species of rodentia. Arch Oral Biol 19:7–11
Hiller CR, Robinson C, Weatherell JA (1975) Variation in the composition of developing rat incisor enamel. Calcif Tissue Res 18:1–12
Kakei M, Nakahara H (1984) Histochemical localization of carbonic anhydrase activity in epiphyseal growth cartilage and calvaria of the rat. Jpn J Oral Biol 26:554–558
Kakei M, Nakahara H (1985) Electroimmunoblotting study of carbonic anhydrase in developing enamel and dentin of the rat incisor. Jpn J Oral Biol 27:357–361
Kondo K, Kuriaki K (1961) Carbonic anhydrase in dental tissue and the effects of parathyroid hormone and fluoride on its activity. J Dent Res 40:971–974
Löwenstam HA, Weiner S (1985) Tranformation of amorphous calcium phosphate to crystalline dahllite in the radular teeth of chitons. Science 227:51–53
Lunt DA, Noble HW (1975) Nature of pigment in teeth of pygmy shrew, Sorex minutus. J Dent Res 54:1087
Marshall AF, Lawless KR (1981) TEM study of the central dark line in enamel crystallites. J Dent Res 60:1773–1782
Miles AEW (1963) Pigmented enamel. Proc R Soc Med 56:918–920
Nakahara H, Kakei M (1984) Central dark line and carbonic anhydrase; problems relating to crystal nucleation in enamel. In: Fearnhead RW, Suga S (eds) Tooth enamel IV. Elsevier, Amsterdam, pp 42–46
Pindborg EV, Pindborg JJ, Plum CM (1946) Studies on incisor pigmentation in relation to liver iron and blood picture in white rat, I. The effect of iron deficiency on the pigmentation of the incisors in rats. Acta Pharmacol 2:285–293
Pindborg JJ (1947) Studies on incisor pigmentation in relation to iron and blood picture in the white rat. Odontol Tidskr 55:443–446
Quint P, Althoff J, Höhling HJ, Boyde A, Laabs WA (1980) Characteristic molar ratios of magnesium, carbon dioxide, calcium and phosphorus in the mineralizing fracture callus and predentine. Calcif Tissue Int 32:257–261
Reith EJ (1961) The ultrastructure of ameloblasts during matrix formation and the maturation of ename. J Biophys Biochem Cytol 9:825–839
Robinson C, Briggs HD, Atkinson PJ, Weatherell JA (1979) Matrix and mineral changes in developing enamel. J Dent Res 58 (B):871–880
Schmidt PF (1984) Localization of trace elements with the laser microprobe mass analyzer (LAMMA). Trace Elements Med 1:13–20
Schmidt PF, Hagen B, Leusmann DB (1985) Laser microprobe mass analysis of carbonate in apatite of biological concretions. In: Armstrong JT (ed) Microbeam analysis-1985. San Francisco Press, San Francisco, pp 331–336
Schmidt WJ (1958) Histologie und Färbung der Zähne des japanischen Riesensalamanders. Z Zellforsch 93:447–450
Selvig KA, Halse A (1975) The ultrastructural localization of iron in rat incisor enamel. Scand J Dent Res 83:88–95
Stein G, Boyle PE (1959) Pigmentation of the enamel of albino rat incisor teeth. Arch Oral Biol 1:97–105
Takano Y, Ozawa H (1981) Cytochemical studies on the ferritin-containing vesicles of the rat incisor ameloblasts with special reference to the acid phosphatase activity. Calcif Tissue Int 33:51–55
Torell P (1955) Iron and dental hard tissues. Odontol Tidskr 63:131–172
Toverud G (1927) Svangerskapets indflydelse paa taendene. Nor Mag Laegevidensk 88:169–185
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Kozawa, Y., Sakae, T., Mishima, H. et al. Electron-microscopic and microprobe analyses on the pigmented and unpigmented enamel of Sorex (Insectivora). Histochemistry 90, 61–65 (1988). https://doi.org/10.1007/BF00495708
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DOI: https://doi.org/10.1007/BF00495708