Summary
K+-dependent p-nitrophenylphosphatase (pNPPase) and Ca++-stimulated adenosine triphosphatase (ATPase) activities were studied in human parotid and submandibular glands using cytochemical methods at the ultrastructural level. In both glands, only the striated-duct epithelium showed K+-pNPPase reaction product, thereby indicating the localization of Na+, K+-ATPase. The precipitate was concentrated on the deep invaginations of the basolateral plasma membranes, in close association with their cytoplasmic surface. Ca++-ATPase activity was also found on the basolateral plasma membranes, but two striking differences from the K+-pNPPase distribution were observed: firstly, Ca++-ATPase appeared in both acinar and ductal cells, and secondly, it was localized on the outer side of the plasma membranes.
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References
Ando T, Fujimoto K, Mayahara H, Miyajima H, Ogawa K (1981) A new one-step method for the histochemistry and cytochemistry of Ca++-ATPase activity. Acta Histochem Cytochem 14:705–726
Bogart BI (1975) Secretory dynamics of the rat submandibular gland. An ultrastructural and cytochemical study of the isoproterenol-induced secretory cycle. J Ultrastruct Res 52:139–155
Bundgaard M, Möller M, Poulsen JH (1977) Localization of sodium pump sites in cat salivary glands. J Physiol 273:339–353
Dupont Y (1983) Is Ca++-ATPase a water pump? FEBS Lett 161:14–20
Ekerdt R, Dahl G, Gratzl M (1981) Membrane fusion of secretory vesicles and liposomes. Two different types of fusion. Biochim Biophys Acta 464:10–22
Ernst SA, Hootman SR (1981) Microscopical methods for the localization of the Na+, K+-ATPase. Histochem J 13:397–418
Furuyama S, Negishi T, Tobe Y, Takiguchi H (1975) Studies on Ca++-stimulated adenosine triphosphatase in rat submandibular glands: its distribution and properties. J Dent Res 54:32–40
Gallacher DV (1982) Are there purinergic receptors on parotid acinar cells? Nature 296:83–86
Garrett JR, Emmelin N (1979) Activities of salivary myoepithelial cells: a review. Med Biol 57:1–28
Gratzl M, Dahl G (1978) Fusion of secretory vesicles isolated from rat liver. J Membr Biol 40:343–364
Han SS, Kim SK, Cho MI (1976) Cytochemical characterization of the myoepithelial cells in palatine glands. J Anat 122:559–570
Lagerlöf F (1983) Effects of flow rate and pH on calcium phosphate saturation in human parotid saliva. Caries Res 17:403–411
Mayahara H, Fujimoto K, Ando T, Ogawa K (1980) A new onestep method for the cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity. Histochemistry 67:125–138
Mayahara H, Ogawa K (1980) Ultracytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in the rat kidney. Acta Histochem Cytochem 13:90–102
Nakagaki I, Goto T, Sasaki S, Imai Y (1978) Histochemical and Cytochemical localization of (Na+-K+) activated adenosine triphosphatase in the acini of dog submandibular glands. J Histochem Cytochem 26:835–845
Pikstaff CA (1980) The cytology of salivary glands. Int Rev Cytol 63:141–261
Publicover SJ, Duncan CJ (1981) Membrane protein mobility and exocytosis. Comp Biochem Physiol 70A:261–263
Riva A, Testa-Riva F, Del Fiacco M, Lantini MS (1976) Fine structure and cytochemistry of the intralobular ducts of the human parotid gland. J Anat 122:627–640
Suddick RP, Dowd FJ (1980) Mechanisms of secretion of saliva. In: Menaker L (ed) The biologic basis of dental caries. Harper and Row, Hagerstown, pp 64–112
Suddick RP, Hyde RJ, Feller RP (1980) Salivary water and electrolytes and oral health. In: Menaker L (ed) The biologic basis of dental caries. Harper and Row, Hagerstown, pp 132–147
Takiguchi H, Son Y, Furuyama S (1974) Separation of calcium-dependent adenosine triphosphatase in the bovine parotid gland from magnesium dependent adenosine triphosphatase. J Dent Res 53:759
Tandler B (1963) Ultrastructure of the human submaxillary gland. II. The base of the striated duct cells. J Ultrastruct Res 9:65–75
Thaysen JH, Thorn NA, Schwartz IL (1954) Excretion of sodium, potassium, chloride and carbon dioxide in human parotid saliva. Am J Physiol 178:155–159
Watson EL, Izutsu KT, Siegel IA (1974) Calcium-stimulated ATPase of dog submandibular gland. Arch Oral Biol 19:13–16
Young JA, Frömter E, Schögel E, Hamann KF (1967) Micropunture and perfusion studies of fluid and electrolyte transport in the rat submaxillary gland. In: Schneyer LH and Schneyer CA (eds) Secretory mechanisms of salivary glands. Academic Press, New York, pp 11–31
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Cossu, M., Lantini, M.S., Puxeddu, P. et al. Cytochemical localization of ouabain-sensitive, K+-dependent p-nitrophenylphosphatase and Ca++-stimulated adenosine triphosphatase activities in human parotid and submandibular glands. Histochemistry 81, 221–225 (1984). https://doi.org/10.1007/BF00495631
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DOI: https://doi.org/10.1007/BF00495631