Skip to main content
SpringerLink
Log in

A simple method for the fluorescence analysis of nucleic acid-dye complexes in cytological preparations

  • Short Communication
  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

This communication describes a simple method for recording fluorescence emission spectra of cytological preparations using a conventional fluorescence spectrophotometer. The emission characteristics of “in situ” complexes between some basic fluorochromes (DAPI, 33258 Hoechst, acridine orange, pyronin Y, and ethidium bromide) and nucleic acid containing structures from smears of chicken blood and Ehrlich tumor cells (chromatin, basophilic cytoplasm) are briefly described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bloch D, Fu CT, Chin E (1978) Flow-cytometric analysis of chromatin. Methods Cell Biol 18:247–276

    Google Scholar 

  • Cañete M, Armas-Portela R, Stockert JC (1983) Cytochemical mechanisms of chromatin fluorescence and staining reactions by thionin solutions. Microsc Acta 87:35–38

    Google Scholar 

  • Cowden RR, Curtis SK (1981) Microfluorometric investigations of chromatin structure. I. Evaluation of nine DNA — specific fluorochromes as probes of chromatin organization. Histochemistry 72:11–23

    Google Scholar 

  • Curtis SK, Cowden RR (1981) Four fluorochromes for the demonstration and microfluorometric estimation of RNA. Histochemistry 72:39–48

    Google Scholar 

  • Dall'Asta R, Marchetti M, Casali E (1981) Complessi ccDNA-DAPI: determinazioni fluorimetriche di alcuni parametri chimico-fisici. Acta Bio Med 52:159–164

    Google Scholar 

  • Espelosin RH, Stockert JC (1982) Neutral red fluorescence of chromatin: specificity and binding mechanism. Z Naturforsch 37 c:139–141

    Google Scholar 

  • Fukuda M, Hoshino K, Naito M, Morita T (1982) A fluorescence cytophotometer operated under computer control for multiparameter cell analysis. Histochemistry 76:1–13

    Google Scholar 

  • Gray JW, Langlois RG, Carrano AV, Burkhart-Schulte K, Van Dilla MA (1979) High resolution chromosome analysis: one and two parameter flow cytometry. Chromosoma 73:9–27

    Google Scholar 

  • Jensen RH, Langlois RG, Mayall BH (1977) Strategies for choosing a deoxyribonucleic acid stain for flow cytometry of metaphase chromosomes. J Histochem Cytochem 25:954–964

    Google Scholar 

  • Jotz MM, Gill JE, Davis DT (1976) A new optical multichannel microspectrofluorometer. J Histochem Cytochem 24:91–99

    Google Scholar 

  • Latt SA (1973) Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Natl Acad Sci USA 70:3395–3399

    Google Scholar 

  • Lee CH, Chang CT, Wetmur JG (1973) Induced circular dichroism of DNA-dye complexes. Biopolymers 12:1099–1122

    Google Scholar 

  • Le Pecq JB, Paoletti C (1967) A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol 27:87–106

    Google Scholar 

  • Magun BE, Kelly JW (1969) A new fluorescent method with phenanthrene-quinone for the histochemical demonstration of arginine residues in tissues. J Histochem Cytochem 17:821–827

    Google Scholar 

  • Moutschen J (1976) Fine structure of chromosomes as revealed by fluorescence analysis. Prog Biophys Mol Biol 31:39–66

    Google Scholar 

  • Nicolini C, Belmont A, Parodi S, Lessin S, Abraham S (1979) Mass action and acridine orange staining: static and flow cytofluorometry. J Histochem Cytochem 27:102–113

    Google Scholar 

  • Ploem JS, de Sterke JA, Bonnet J, Wasmund H (1974) A micro-spectrofluorometer with epi-illumination operated under computer control. J Histochem Cytochem 22:668–677

    Google Scholar 

  • Rhys Williams AT (1980) Fluorescence detection in liquid chromatography. Perkin-Elmer FL Publications, England

    Google Scholar 

  • Shapiro HM (1981) Flow cytometric estimation of DNA and RNA content in intact cells stained with Hoechst 33342 and Pyronin Y. Cytometry 2:143–150

    Google Scholar 

  • Stockert JC, Molero ML, Espelosin RH (1983) Fluorescence patterns of chromatin and cytoplasm by hematoxylin solutions. Z Naturforsch 38c:153–155

    Google Scholar 

  • Tanke HJ, Rothbarth PH, Vassen JMJJ, Koper GJM, Ploem JS (1983) Flow cytometry of reticulocytes applied to clinical hematology. Blood 61:1091–1097

    Google Scholar 

  • Tas J, Westerneng G (1981) Fundamental aspects of the interaction of propidium diiodide with nucleic acids studied in a model system of polyacrylamide films. J Histochem Cytochem 29:929–936

    Google Scholar 

  • Taylor IW, Milthorpe BK (1980) An evaluation of DNA fluorochromes, staining techniques and analysis for flow cytometry. J Histochem Cytochem 28:1224–1232

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Citología e Histología, Facultad de Ciencias, Universidad Autonoma de Madrid, 34, Madrid, Spain

    A. Villanueva, J. C. Stockert & R. Armas-Portela

Authors
  1. A. Villanueva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. C. Stockert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Armas-Portela
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Villanueva, A., Stockert, J.C. & Armas-Portela, R. A simple method for the fluorescence analysis of nucleic acid-dye complexes in cytological preparations. Histochemistry 81, 103–104 (1984). https://doi.org/10.1007/BF00495409

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00495409

Keywords

Search

Navigation