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Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections

IV. Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (decarboxylating)

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Summary

Improved histochemical techniques for the demonstration of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (decarboxylating) in tissue sections are described. With these techniques a semipermeable membrane is interposed between the incubating solutions and the tissue sections preventing diffusion of enzymes into the medium during incubation. In the histochemical system the NADP+-dependent enzymes catalyze the electron transfer from D-glucose 6-phosphate or 6-phospho-D-gluconate into NADP+. Phenazine methosulphate and menadione serve as intermediate electron acceptors between reduced coenzyme and nitro-BT. Sodiumazide is incorporated into the incubating-medium to block electron transfer to the cytochromes. Problems involved in the histochemical demonstration of both enzymes are discussed.

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Authors and Affiliations

  1. Laboratory of Pathological Anatomy, Histochemical and Biochemical Section, University of Amsterdam, The Netherlands

    A. E. F. H. Meijer & G. P. de Vries

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  1. A. E. F. H. Meijer
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  2. G. P. de Vries
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Meijer, A.E.F.H., de Vries, G.P. Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. Histochemistry 40, 349–359 (1974). https://doi.org/10.1007/BF00495042

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