Summary
The occurrence of endogenous lectins in the ovaries of four fish species has been studied by indirect immunofluorescence staining with antibodies against individual lectins. Paraffin sections of the ovary of perch (Perca fluviatilis L.) were treated with an antibody against perch lectin. In cryostat sections of the tench (Tinca tinca L.) ovary, the l-rhamnose-specific lectin “I” was detected with a specific antibody. In cryostat sections of both roach (Rutilus rutilus L.) and rudd (Scardinius erythrophthalmus L.) ovaries, lectins were localized using a single antibody against roach lectin. The isolation of tench lectins is briefly described. In the fish species employed for this study, lectins are associated exclusively with the content and surrounding membrane of cortical vesicles situated within the cytoplasm of maturing oocytes. The positive reaction with lectin antibody was observed almost immediately after the formation of the first cortical vesicles in the peripheral cytoplasm of early previtellogenic oocytes. Their lectin content increases during the later stages when cortical granules fill the whole cytoplasm before moving towards the cell periphery, as the oocyte starts to accumulate yolk. The presence of lectins within cortical vesicles is significant also in view of the polysaccharide content of these structures. In the vitellogenic oocytes lectins seem to move towards the cell periphery and accumulate beneath the plasma membrane. Our observations are discussed in view of the present ideas on the intracellular function of lectins, and with respect to the role of cortical vesicles in fertilization and in postfertilization modifications of the egg envelopes.
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References
Arndt EA (1960) Über die Rindenvakuolen der Teleosteeroozyten. Z Zellforsch 51:209–224
Devillers C (1961) Structural and dynamic aspects of the development of the teleostean egg. Adv Morphogen 1:379–428
Filka K, Čoupek J, Kocourek J (1978) Studies on lectins. XL. O-Glycosyl derivatives of Spheron in affinity chromatography of lectins. Biochim Biophys Acta 539:518–528
Filka K, Čoupek J, Kocourek J (1979) Glycosyl derivatives of Separon and their use in isolation and immobilization of leotins. In: Peeters H (ed) Protides of biological fluids. 27th Colloquium. Pergamon Press, Oxford, New York, pp 375–380
Ginsburg AS (1961) The block to polyspermy in sturgeon and trout with special refenence to the role of cortical granules (alveoli). J Embryol Exp Morphol 9:173–190
Hagiwara S, Jaffe LA (1979) Electrical properties of egg cell membranes. Annu Rev Biophys Bioeng 8:385–416
Hořejší V, Kocourek J (1973) Studies on phytohemagglutinins. XII. O-Glycosyl polyacrylamide gels for affinity chromatography of phytohemagglutinins. Biochim Biophys Acta 297:346–351
Hořejší J, Smetana R (1956) The isolation of gamma globulin from blood-serum by rivanol. Acta Med Scand 155:65–70
Köhler W (1970) Reaktionen von Mikroorganismen mit Protektinen. In: Perlick E, Pleuert W, Prokop O (eds) Fortschritte der Hämatologie. JA Barth, Leipzig, pp 41–70
Kothbauer H, Schenkel-Brunner H (1974) Hemagglutinins in female fish gonads: A discussion of their biological function — inhibition tests with male gonads. Z Immunitätsforsch Allerg Klin Immunol 147:89–90
Krajhanzl A (1977) Some lectins from fish roe, RNDr thesis. Fac Sciences, Charles Univ, Praha (in Czech)
Krajhanzl A, Kocourek J (1982) Properties of the lectin from oocyte cortical granules of the perch (Perca fluviatilis). Change in dependence of the degree of maturity of oocytes. In: Bøg-Hansen C, Spengler A (eds) Abstr 5th Int Lectin Meeting. W de Gruyter, Berlin, pp 104–105
Krajhanzl A, Hořejší V, Kocourek J (1978) Studies on lectins. XLII. Isolation, partial characterization and comparison of lectins from the roe of five fish species. Biochim Biophys Acta 532:215–224
Nosek J, Krajhanzl A, Kocourek J (1982) Subcellular localization of an endogenous lectin in fish oocytes. In: Bøg-Hansen C, Spengler A (eds) Abstr 5th Int Lectin Meeting. W de Gruyter, Berlin, pp 134–135
Olden K, Parent JB, White SL (1982) Carbohydrate moieties of glycoproteins. A re-evaluation of their function. Biochim Biophys Acta 650:209–232
Prokop O, Uhlenbruck G (1970) Protektine. In: Perlick E, Pleuert W, Prokop O (eds) Fortschritte der Hämatologie. JA Barth, Leipzig, pp 17–40
Prokop O, Schlesinger D, Geserick G (1967a) Thermostabiles B-Agglutinin aus Konserven von Lachskaviar. Z Immunitätsforsch Allerg Klin Immunol 132:491–494
Prokop O, Schnitzler S, Uhlenbruck G (1967b) Über einen kräftigen H-antikörper bei zwei Vertretern der Percidae, aufgefunden im Rogen der Tiere. Acta Biol Med Germ 18:K7-K9
Prokop O, Uhlenbruck G, Rothe A, Cohen E (1974) Protectins: past, present problems and perspectives. Ann NY Acad Sci 234:228–231
Schenkel-Brunner H, Kothbauer H (1978) Hemagglutinins in female fish gonads. Comparative investigations on perch (Perca fluviatilis) gonads of different stages of development. Z Immunitätsforsch 154:155–161
Schnitzler S, Müller G, Prokop O (1967) Ein “neuer” Antikörper: anti Prut aufgefunden im Rogen von Rutilus rutilus. Z Immunitätsforsch Allerg Klin Immunol 134:45–53
Yamamoto K (1956a) Studies on the formation of fish eggs. I. Annual cycle in the development of ovarian eggs in the flounder, Liopsetta obscura. J Fac Sci Hokkaido Univ Ser IV, 12:363–374
Yamamoto K (1956b) Studies on the formation of fish eggs. III. Localization of polysaccharides in oocytes of Liopsetta obscura. J Fac Sci Hokkaido Univ Ser IV, 12:391–400
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Nosek, J., Krajhanzl, A. & Kocourek, J. Studies on lectins. Histochemistry 79, 131–139 (1983). https://doi.org/10.1007/BF00494350
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DOI: https://doi.org/10.1007/BF00494350