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DNA-fluorometry of mammalian sperm

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Summary

The DNA-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescence distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature sperm with a coefficient of variation below 2%.

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Authors and Affiliations

  1. Abteilung für experimentelle Tumorforschung der Fachklinik Hornheide, Universität Münster, D-4400, Münster, Germany

    Jürgen Zante, Johannes Schumann, Wolfgang Göhde & Ursula Hacker

Authors
  1. Jürgen Zante
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  2. Johannes Schumann
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  3. Wolfgang Göhde
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  4. Ursula Hacker
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Zante, J., Schumann, J., Göhde, W. et al. DNA-fluorometry of mammalian sperm. Histochemistry 54, 1–7 (1977). https://doi.org/10.1007/BF00493324

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  • DOI: https://doi.org/10.1007/BF00493324

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