Summary
The oxyntic mucosa of the dog stomach is rich in cells storing pancreatic-type glucagon. The cells were identified by immunocytochemistry and found to be indistinguishable from pancreatic A cells in the electron microscope. Ultrastructural identification of the immunoreactive cells was accomplished by the use of consecutive semithin-ultrathin sections, a technique that permitted the use of optimally fixed material.
Similar content being viewed by others
References
Baetens, D., Rufener, C., Srikant, B.C., Dobbs, R., Unger, R., Orci, L.: Identification of glucagon-producing cells (A cells) in dog gastric mucosa. J. Cell Biol. 69, 455–464 (1976a)
Baetens, D., Rufener, C., Unger, R.H., Renold, A.E., Orci, L.: Identification par immunocytochimie de le cellule sécrétant le glucagon dans la muqueuse gastrique. C.R. Acad. Sci. (Paris) 282, 195–197 (1976b)
Beauvillain, J.-C., Tramu, G., Dubois, M.P.: Characterization by different techniques of adrenocorti-cotropin and gonadotropin producing cells in Lerot pituitary (Eliomys quercinus). Cell Tiss. Res. 158, 301–317 (1975)
Grimelius, L., Capella, C., Buffa, R., Polak, J.M., Pearse, A.G.E., Solcia, E.: Cytochemical and ultrastructural differentiation of enteroglucagon and pancreatic-type glucagon cells of the gastrointestinal tract. Virchows Arch. Abt. B Cell Path. 20, 217–228 (1976)
Holst, J., Aasted, B.: Production and evaluation of glucagon antibodies for radioimmunoassay. Acta endocr. (Kbh.) 77, 715–726 (1974)
Kubes, L., Jirasek, K., Lomsky, R.: Endocrine cells of the dog gastrointestinal mucosa. Cytologia (Tokyo) 39, 179–194 (1974)
Larsson, L.-I., Holst, J., Håkanson, R., Sundler, F.: Distribution of glucagon immunoreactivity in the digestive tract of various mammals: an immunohistochemical and immunochemical study. Histochemistry 44, 281–290 (1975)
Mayor, H.D., Hampton, J.C., Rosario, R.: A simple method for removing the resin from epoxyembedded tissue. J. biophys. Cytol. 9, 909–910 (1961)
Sasaki, H., Rubalcava, B., Baetens, D., Blazquez, E., Srikant, C.B., Orci, L., Unger, R.H.: Identification of glucagon in the gastrointestinal tract. J. clin. Invest. 56, 135–145 (1975)
Solcia, E., Capella, C., Vassallo, G., Buffa, R.: Endocrine cells of the gastric mucosa. Int. Rev. Cytol. 42, 223–286 (1975)
Solcia, E., Pearse, A.G.E., Grube, D., Kobayashi, S., Bussolati, G., Creutzfeldt, W., Gepts, W.: Revised Wiesbaden classification of gut endocrine cells. Rendic. Gastroent. 5, 13–16 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sundler, F., Alumets, J., Holst, J. et al. Ultrastructural identification of cells storing pancreatic-type glucagon in dog stomach. Histochemistry 50, 33–37 (1976). https://doi.org/10.1007/BF00492783
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00492783