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Ultrastructural identification of cells storing pancreatic-type glucagon in dog stomach

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Summary

The oxyntic mucosa of the dog stomach is rich in cells storing pancreatic-type glucagon. The cells were identified by immunocytochemistry and found to be indistinguishable from pancreatic A cells in the electron microscope. Ultrastructural identification of the immunoreactive cells was accomplished by the use of consecutive semithin-ultrathin sections, a technique that permitted the use of optimally fixed material.

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References

  • Baetens, D., Rufener, C., Srikant, B.C., Dobbs, R., Unger, R., Orci, L.: Identification of glucagon-producing cells (A cells) in dog gastric mucosa. J. Cell Biol. 69, 455–464 (1976a)

    Google Scholar 

  • Baetens, D., Rufener, C., Unger, R.H., Renold, A.E., Orci, L.: Identification par immunocytochimie de le cellule sécrétant le glucagon dans la muqueuse gastrique. C.R. Acad. Sci. (Paris) 282, 195–197 (1976b)

    Google Scholar 

  • Beauvillain, J.-C., Tramu, G., Dubois, M.P.: Characterization by different techniques of adrenocorti-cotropin and gonadotropin producing cells in Lerot pituitary (Eliomys quercinus). Cell Tiss. Res. 158, 301–317 (1975)

    Google Scholar 

  • Grimelius, L., Capella, C., Buffa, R., Polak, J.M., Pearse, A.G.E., Solcia, E.: Cytochemical and ultrastructural differentiation of enteroglucagon and pancreatic-type glucagon cells of the gastrointestinal tract. Virchows Arch. Abt. B Cell Path. 20, 217–228 (1976)

    Google Scholar 

  • Holst, J., Aasted, B.: Production and evaluation of glucagon antibodies for radioimmunoassay. Acta endocr. (Kbh.) 77, 715–726 (1974)

    Google Scholar 

  • Kubes, L., Jirasek, K., Lomsky, R.: Endocrine cells of the dog gastrointestinal mucosa. Cytologia (Tokyo) 39, 179–194 (1974)

    Google Scholar 

  • Larsson, L.-I., Holst, J., Håkanson, R., Sundler, F.: Distribution of glucagon immunoreactivity in the digestive tract of various mammals: an immunohistochemical and immunochemical study. Histochemistry 44, 281–290 (1975)

    Google Scholar 

  • Mayor, H.D., Hampton, J.C., Rosario, R.: A simple method for removing the resin from epoxyembedded tissue. J. biophys. Cytol. 9, 909–910 (1961)

    Google Scholar 

  • Sasaki, H., Rubalcava, B., Baetens, D., Blazquez, E., Srikant, C.B., Orci, L., Unger, R.H.: Identification of glucagon in the gastrointestinal tract. J. clin. Invest. 56, 135–145 (1975)

    Google Scholar 

  • Solcia, E., Capella, C., Vassallo, G., Buffa, R.: Endocrine cells of the gastric mucosa. Int. Rev. Cytol. 42, 223–286 (1975)

    Google Scholar 

  • Solcia, E., Pearse, A.G.E., Grube, D., Kobayashi, S., Bussolati, G., Creutzfeldt, W., Gepts, W.: Revised Wiesbaden classification of gut endocrine cells. Rendic. Gastroent. 5, 13–16 (1973)

    Google Scholar 

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Sundler, F., Alumets, J., Holst, J. et al. Ultrastructural identification of cells storing pancreatic-type glucagon in dog stomach. Histochemistry 50, 33–37 (1976). https://doi.org/10.1007/BF00492783

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  • DOI: https://doi.org/10.1007/BF00492783

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