Summary
Secretory epithelia and ductular glands were studied in main pancreatic ducts of guinea pig, rabbit, rat, and man. Brunner glands were studied for comparison. Semithin sections from Epon-embedded tissues were etched with sodium methylate and incubated with horseradish peroxidase-conjugated lectins. Columnar cells in the epithelium of pancreatic ducts are endowed with well-developed microvillar borders. These apical regions strongly stain with Lotus A-, wheat germ-, and Ricinus I-lectins. Basolateral plasma membranes bind Ricinus I-, Ulex europaeus I-, and wheat germ-lectins. Cytosomes in the supranuclear regions of epithelial cells are interpreted as secretory granules. These droplets are marked by wheat germ-lectin and to a lesser degree by Ricinus I- and Ulex europaeus I-lectins. Ductular glands of the main pancreatic ducts contain secretions that bind Helix-, wheat germ-, and Ulex europaeus I-lectins. Their apical and basolateral cell membranes deeply stain with wheat germ- and Ulex europaeus I-lectins. Secretions of Brunner glands bind Ricinus I-, Ulex europaeus I-, Helix-, and wheat germ lectins. Their apical and basolateral cell membranes stain with Ricinus I- and wheat germ-lectins.-Species differences in lectin-binding affinities of complex carbohydrates were observed and are described.
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Part of these results has been presented as a poster during the “4th Symposium on Lectins in Cell Biology and Medicine” June 25, 1983, Cologne, Federal Republic of Germany
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Geleff, S., Böck, P. Pancreatic duct glands. II. Lectin binding affinities of ductular epithelium, ductular glands, and Brunner glands. Histochemistry 80, 31–38 (1984). https://doi.org/10.1007/BF00492768
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DOI: https://doi.org/10.1007/BF00492768