Summary
Sperm antigen expression during epididymal transit was examined in 4- to 16-week-old intact and castrated ICR mice, using the avidin-biotin complex (ABC) immunohistochemical method with monoclonal antibody T21 against a flagellar surface antigen. On untreated sections, the antigen was first expressed weakly on sperm in the proximal part of the corpus epididymis, and intraluminal components were stained in 4-week-old mice. Epididymal epithelial cells and their stereocilia, and cells in other reproductive organs were not stained. In contrast, on sections treated with neuramainidase, (1) the initial site of antigen appearance is a more proximal position in treated than in untreated sections, (2) stereocilia stained strongly, (3) the staining intensity of sperm and intraluminal components increased, and (4) some clear cells in the epithelium from the distal position of the caput to the corpus epididymis were stained. These results indicate that the antigen is produced by clear cells of the epididymal epithelium, that the antigenic determinant is masked initially by sialic acid residues, and that expression of the antigenic determinant on the sperm surface during epididymal maturation apparently involves desialylation.
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References
Bedford JM (1963) Changes in the electrophoretic properties of rabbit spermatozoa during passage through the epididymis. Nature 200:1178–1180
Bedford JM, Calvin HI, Copper GW (1973) The maturation of spermatozoa in the human epididymis. J Reprod Fertil (Suppl) 18:199–213
Brooks DE (1983) Selective binding of specific rat epididymal secretory proteins to spermatozoa and erythrocytes. Gamete Res 4:367–376
Czuppon AB (1984) Biochemical characterization of a human spermatozoal sialoglycoprotein with respect to antigenicity masking by its sialic acid moieties. Biochem Int 8:9–18
Dacheux JL, Paquignon M (1980) Relations between the fertilizing ability, motility and metabolism of epididymal spermatozoa. Reprod Nutr Dev 20 (4A):1085–1099
Eddy EM, Vernon RB, Muller CH, Hahnel AC, Fenderson BA (1985) Immunodissection of sperm surface modifications during epididymal maturation. Am J Anat 174:225–237
Feuchter FA, Green MF, Tabet AJ (1987) Maturation antigen of the mouse sperm flagellum: II. Origin from holocrine cells of the distal caput epididymis. Anat Rec 217:146–152
Hoffmann DS, Killian GJ (1981) Isolation of epithelial cells from the corpus epididymis and analysis for glyceryl phosphorylcholine, sialic acid, and protein. J Exp Zool 217:93–102
Kelm S, Shukla AK, Paulson JC, Schauer R (1986) Reconstitution of the masking effect of sialic acid groups of sialidase-treated erythrocytes by the action of sialyltransferases. Carbohydr Res 149:59–64
Kopecný V, Fléchon JE, Pivko J (1984) Binding of secreted glycoproteins to spermatozoa in the mammalian epididymis: A finestructure autoradiographic study. Anat Rec 208:109–206
Moore HDM (1979) The net negative surface charge of mammalian spermatozoa as determined by isoelectric focusing. Changes following sperm maturation, ejaculation, incubation in the female tract, and after enzyme treatment. Int J Androl 2:244–262
Orgebin-Crist MC, Fournier-Delpech S (1982) Sperm-egg interaction: evidence for maturational changes during epididymal transit. J Androl 3:429–433
Saling PM (1982) Development of the ability to bind to zonal pellucidae during epididymal maturation: reversible immobilization of mouse spermatozoa by lanthanum. Biol Repord 26:429–436
Schauer R (1987) Biochemistry of sialic acids. Seikagaku 59:133–146
Smith CA, Hartman TD, Moore HDM (1986) A determinant of Mr 34,000 expressed by hamster epididymal epithelium binds specifically to spermatozoa in co-culture. J Reprod Fertil 78:337–345
Toowicharanont P, Chulavatnatol M (1983) Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymis. J Reprod Fertil 67:275–280
Toshimori K, Eddy EM (1985) Mouse sperm surface maturation antigens of 54 Kd and 34 Kd recognized by a monoclonal antibody. J Cell Biol 101:363a (abstract)
Toshimori H, Toshimori K, Ōura C, Matsuo H (1987) Immunohistochemistry and immunocytochemistry of atrial natriuretic polypeptide in porcine heart. Histochemistry 86:595–601
Toshimori K, Ōura C (1988) Secreted sialic acid influences sperm surface antigen expression during epididymal passage in the mouse. Proc Kagoshima Int Symposium Glycoconjugates in Medicine 1:108–112
Vernon RB, Muller CH, Herr JC, Feuchter FA, Eddy EM (1982) Epididymal secretion of a mouse sperm surface component recognized by a monoclonal antibody. Biol Reprod 26:523–535
Vernon RB, Muller CH, Eddy EM (1987) Further characterization of a secreted epididymal glycoprotein in mice that binds to sperm tails. J Androl 8:123–128
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Toshimori, K., Araki, S. & Ōura, C. Masking of sperm maturation antigen by sialic acid in the epididymis of the mouse. Histochemistry 90, 195–200 (1988). https://doi.org/10.1007/BF00492507
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DOI: https://doi.org/10.1007/BF00492507