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The distribution of sympathetic adrenergic, tyrosine hydroxylase- and neuropeptide Y-immunoreactive nerves in human axillary sweat glands

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Summary

The innervation of human axillary sweat glands was studied by using the specific SPG (sucrose-potassium phosphate-glyoxylic acid) catecholamine histofluorescence method and the peroxidase-antiperoxidase (PAP) immunocytochemical method. The present results demonstrated that human sweat glands are surrounded by nerves containing a weak tyrosine hydroxylase activity. Nerves showing catecholamine histofluorescence could be visualized around the sweat glands only in the presence of exogenous catecholamine (adrenaline in the local anestheticum). In all tissue specimens studied fluorescent adrenergic nerves could be seen around arteries and arterioles corresponding to the distribution of neuropeptide Y-like immunoreactive nerves.

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References

  • Björklund H, Hökfelt T, Goldstein M, Terenius L, Olson L (1985) Appearance of the noradrenergic markers tyrosine hydroxylase and neuropeptide Y in cholinergic nerves of the iris following sympathectomy. J Neurosci 5:1633–1643

    Google Scholar 

  • Dale HH, Feldberg W (1934) The chemical transmission of secretory impulses to the sweat glands of the cat. J Physiol 82:121–128

    Google Scholar 

  • Hartschuh W, Weihe E, Reinecke M (1983) Peptidergic (neurotensin, VIP, substance P) nerve fibers in the skin. Immunohistochemical evidence for an involvement of neuropeptides in nociception, pruritus and inflammation. Br J Dermatol 109 (Suppl 25):14–17

    Google Scholar 

  • Hartschuh W, Reinecke M, Weihe E, Yanaihara N (1984) VIP-immunoreactivity in the skin of various mammals: Immunohistochemical, radioimmunological and experimental evidence for a dual localization in cutaneous nerves and Merkel cells. Peptides 5:239–254

    Google Scholar 

  • Landis SC (1983) Development of cholinergic sympathetic neurons: evidence for transmitter plasticity in vivo. Fed Proc 42:1633–1638

    Google Scholar 

  • Landis SC, Keefe D (1983) Evidence for neurotransmitter plasticity in vivo: Developmental changes in properties of cholinergic sympathetic neurons. Dev Biol 98:349–372

    Google Scholar 

  • Lundberg JM, Hökfelt T, Schultzberg M, Uvnäs-Wallenstein K, Köhler C, Said SI (1979) Occurrence of vasoactive intestinal polypeptide (VIP)-like immunoreactivity in certain cholinergic neurons of the cat: Evidence from combined immunohistochemistry and acetylcholinesterase staining. Neuroscience 4:1539–1559

    Google Scholar 

  • Lundberg JM, Terenius L, Hökfelt T, Martling CR, Tatemoto K, Mutt V, Polak J, Bloom S, Goldstein M (1982) Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand 116:477–480

    Google Scholar 

  • Lundberg JM, Saria A, Änggård A, Hökfelt T, Terenius L (1984) Neuropeptide Y and noradrenaline interaction in peripheral cardiovascular control. Clin Exp Hypertens (A) 6:1961–1972

    Google Scholar 

  • Mazurkiewicz JE, Nakane PK (1972) Light and electron microscopic localization of antigens in tissue embedded in polyethylene glycol with a peroxidase labelled antibody method. J Histochem Cytochem 20:969–974

    Google Scholar 

  • Pickel VM, Joh TH, Reis DJ, Leeman SE, Miller RJ (1979) Electron microscopic localization of substance P and enkephalin in axon terminals related to dendrites of catecholaminergic neurons. Brain Res 160:387–400

    Google Scholar 

  • Rechardt L, Waris T, Rintala A (1976) Innervation of human axillary sweat glands. Scand J Plast Reconstr Surg 10:107–112

    Google Scholar 

  • Siegel RE, Schwab M, Landis SC (1982) Developmental changes in the neurotransmitter properties of cholinergic sympathetic neurons in vivo. Soc Neurosci Abstr 8:7

    Google Scholar 

  • Sokolov VE, Shabadash SA, Zelikina TI (1981) Innervation of the eccrine sweat glands. Biol Bull Acad Sci USSR 7:331–346

    Google Scholar 

  • Sokolov VE, Shabadash SA, Zelikina TI (1982) Innervation of the apocrine sweat glands. Biol Bull Acad Sci USSR 8:242–254

    Google Scholar 

  • Sternberger LA (1979) Immunohistochemistry. 2nd edn. John Wiley & Sons, New York

    Google Scholar 

  • Sternini C, Brecha N (1985) Distribution and colocalization of neuropeptide Y- and tyrosine hydroxylase-like immunoreactivity in the guinea-pig heart. Cell Tissue Res 241:93–102

    Google Scholar 

  • Tatemoto K (1982) Neuropeptide Y. The complete amino acid sequence of the brain peptide. Proc Natl Acad Sci USA 79:5485–5489

    Google Scholar 

  • Torre JC de la (1980) An improved approach to histofluorescence using the SPG method for tissue monoamines. J Neurosci Methods 3:1–5

    Google Scholar 

  • Uno H (1977) Sympathetic innervation of the sweat glands and piloerector muscles of macaques and human beings. J Invest Dermatol 69:112–120

    Google Scholar 

  • Vaalasti A, Tainio H, Rechardt L (1985) Vasoactive intestinal polypeptide (VIP)-like immunoreactivity in the nerves of human axillary sweat glands. J Invest Dermatol 85:246–248

    Google Scholar 

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Tainio, H., Vaalasti, A. & Rechardt, L. The distribution of sympathetic adrenergic, tyrosine hydroxylase- and neuropeptide Y-immunoreactive nerves in human axillary sweat glands. Histochemistry 85, 117–120 (1986). https://doi.org/10.1007/BF00491757

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  • DOI: https://doi.org/10.1007/BF00491757

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