Abstract
Somatic hybrids of drug-resistant mutant hamster and mouse cell lines have been isolated and propagated in long-term culture and have been studied in respect to karyotype and three enzymes. During the course of propagation the long-surviving hybrid clones show progressive loss of telocentric chromosomes associated in at least one case with loss of mouse enzyme. Hybrid clones showed hybrid molecules for malate dehydrogenase (MDH), lactate dehydrogenase (LDH), and 6-phosphogluconate dehydrogenase (6PGD) made up by recombination of parental subunits.
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Barski, G., Sorieul, S., and Cornefert, F. (1960). Production dans des cultures in vitro de deux souches cellulaires en association, de cellules de caractère “hybride.” Compt. Rend. 251 1825.
Davidson, R. L., Ephrussi, B., and Yamamoto, K. (1966). Regulation of pigment synthesis in mammalian cells as studied by somatic hybridization. Proc. Natl. Acad. Sci. (U.S.) 56 1437.
Ephrussi, B., Stenchever, M. A., and Scarletta, L. J. (1964). In Second International Congress on Congenital Malformations. Edited by M. Fishbein. International Medical Congress, Ltd., New York, p. 85.
Harris, H., and Watkins, J. F. (1965). Hybrid cells derived from mouse and man. Artificial heterokaryons of mammalian cells from different species. Nature 205 640.
Hsu, T. C. (1964). Registry of animal cell lines certified by the cell culture collection committee. 1st ed.
Littlefield, J. W. (1964). Three degrees of guanylic acid-inosinic acid pyrophosphorylase deficiency in mouse fibroblasts. Nature 203 1142.
Littlefield, J. W. (1966). The use of drug-resistant markers to study the hybridization of mouse fibroblasts. Exptl. Cell Res. 41 190.
Littlefield, J. W., and Basilico, C. (1966). Infection of thymidine kinase deficient BHK cells with polyoma virus. Nature 211 250.
Scarletta, L. J., Rushforth, N. B., and Ephrussi, B. (1967). Isolation and properties of hybrids between somatic mouse and Chinese hamster cells. Genetics 57 107.
Smithies, O. (1955). Zone electrophoresis in starch gels: Group variations in the serum proteins of normal adults. Biochem. J. 61 629.
Weiss, M. C., and Ephrussi, B. (1966a). Studies of interspecific (rat × mouse) somatic hybrids. I. Isolation, growth and evolution of the karyotype. Genetics 54 1095.
Weiss, M. C., and Ephrussi, B. (1966b). Studies of interspecific (rat × mouse) somatic hybrids. II. Lactic dehydrogenase and β-glucuronidase. Genetics 54 1111.
Weiss, M. C., and Green, H. (1967). Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc. Natl. Acad. Sci. (U.S.) 58 1104.
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This work was supported by National Institutes of Health Grant HD 00486.
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Migeon, B.R. Hybridization of somatic cells derived from mouse and Syrian hamster: Evolution of karyotype and enzyme studies. Biochem Genet 1, 305–322 (1968). https://doi.org/10.1007/BF00491487
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DOI: https://doi.org/10.1007/BF00491487