Summary
Epithelial cells of eight human gallbladders with cholesterosis were examined. In the supranuclear portion of the epithelial cells of one case, many spicular, circular and plate-like pale crystalline structures are present. Spicular and circular structures have not a limiting membrane, but plate-like structures are apparently bounded by a limiting membrane that clearly shows trilamellar structure. After digitonin treatment, the spicular and circular crystalline structures become denser. On the other hand, the plate-like structures do not become denser by digitonization. In the epithelial cells that contain no crystalline structure, there also occur many reaction precipitates after digitonization. These findings may suggest that free cholesterol is highly present in the epithelial cells of gallbladder with cholesterosis and that it, in some case, precipitates in the form of spicular or circular structure for the rapid fixation process.
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References
Boyd, W.: Studies in gallbladder pathology. Brit. J. Surg. 10, 337–356 (1922)
Ganguly, J., Murthy, S.K.: Absorption of cholesterol and vitamin A in rats. In Proceedings of an intestinal symposium of lipid transport. (Meng, H. C., ed.), p. 22–32. Charles C. Thomas Publisher, Springfield/Ill.: 1964
Nakayama, F., Johnston, C. G.: Fractionation of bile lipids with silicic acid column chromatography. J. Lab. clin. Med. 59, 364–370 (1962)
Ökrös, J.: Digitonin reaction in electron microscopy. Histochemie 13, 91–96 (1968)
Scallen, T. J., Dietert, S. E.: The quantitative retention of cholesterol in mouse liver prepared for electron microscopy by fixation in a digitonin-containing aldehyde solution. J. Cell Biol. 40, 802–813 (1969)
Williamson, J. R.: Ultrastructural localization and distribution of free cholesterol (3β-hydroxysterols) in tissue. J. Ultrastruct. Res. 27, 118–133 (1969)
Windaus, A.: Über die quantitative Bestimmung des Cholesterins und der Cholesterinester in einigen normalen und pathologischen Nieren. Hoppe-Seyler Z. physiol. chem. 65, 110–117 (1910)
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Koga, A., Todo, S. & Nishimura, M. Electron microscopic observations on the cholesterol distributed in the epithelial cells of the gallbladder. Histochemistry 44, 303–306 (1975). https://doi.org/10.1007/BF00490366
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DOI: https://doi.org/10.1007/BF00490366