Summary
The specificity of anti-nucleic acid antisera can immunocytochemically be evaluated with test systems which apply various nucleic acids immobilized to inert matrices. When using polyrA.polyrU as a model compoud for dsRNA, it is important to prevent the formation of the triple stranded form polyrA.(polyrU)2.
Anti-dsRNA antibodies which, when tested with the correct test system, proved to be present in an earlier described anti-DNA.RNA serum, could be removed by adsorption. By cytofluorometric comparison of the immunofluorescence signals obtained with the anti-dsRNA containing serum and the absorbed serum, it could be shown that the anti-dsRNA antibodies do not contribute to the specific signals measured after in situ hybridization.
Repetitive incubations with the anti-DNA.RNA serum led to a considerable increase in immunofluorescence signal.
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This work was subsidized in part by Het Praeventiefonds, The Hague, The Netherlands
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Raap, A.K., Marijnen, J.G.J. & van der Ploeg, M. Anti-DNA.RNA sera. Histochemistry 81, 517–520 (1984). https://doi.org/10.1007/BF00489529
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DOI: https://doi.org/10.1007/BF00489529