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Thin-layer chromatography of radioactively labelled cholesterol and precursors from biological material

Dünnschicht-Chromatographie von radioaktiv markiertem Cholesterin und Vorstufen aus biologischem Material. Eine einfache und empfindliche Methode zur Untersuchung einer Beeinflussung des Sterolbiosynthesewegs

A simple and sensitive method for investigating actions on the sterol pathway

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  • Biochemical/clinical Analyses
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Summary

The investigation methods of the action of xenobiotics on sterol biosynthesis from 14C-acetate in rat hepatocyte cultures can be developed, with regard to extraction using Extrelut and the separation of the sterol pattern by thin-layer chromatography, in such a way that they are suitable for wider application, e.g., screening. Good visualisation and recognition of changes in the sterol pattern are possible using autoradiography of the thin-layer chromatogram.

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Authors and Affiliations

  1. Departments of Medical and Chemical Research, Boehringer Mannheim GmbH, Sandhofer Strasse 116, D-6800, Mannheim, Federal Republic of Germany

    J. Pill, J. Aufenanger, K. Stegmeier, F. H. Schmidt & D. Müller

Authors
  1. J. Pill
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  2. J. Aufenanger
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  3. K. Stegmeier
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  4. F. H. Schmidt
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  5. D. Müller
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Dedicated to Dr. E. Roesch on occasion of his 65th birthday

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Pill, J., Aufenanger, J., Stegmeier, K. et al. Thin-layer chromatography of radioactively labelled cholesterol and precursors from biological material. Z. Anal. Chem. 327, 558–560 (1987). https://doi.org/10.1007/BF00487248

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  • DOI: https://doi.org/10.1007/BF00487248

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