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Respiratory-deficient, yeastlike mutant of Mucor

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Abstract

Stable mutants of Mucor bacilliformis having lost the ability to grow filamentously and to sporulate occur spontaneously with a frequency of about one in every 3000 colonies. On solid and in liquid medium these mutants have a typical yeastlike morphology and reproduce by budding. The detailed study of one of these mutants shows that the inability to form filaments and spores is accompanied by the loss of cytochrome oxidase activity. This mutant is unable to take up oxygen but has a high level of alcoholic fermentation, which appears to be the major if not the sole source of energy.

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References

  • Bartnicki-Garcia, S. (1968). Control of dimorphism in Mucor by hexoses: Inhibition of hyphal morphogenesis. J. Bacteriol. 96 1586.

    Google Scholar 

  • Bartnicki-Garcia, S., and Nickerson, W. J. (1961). Thiamine and nicotinic acid: Anaerobic growth factors for Mucor rouxii. J. Bacteriol. 82 142.

    Google Scholar 

  • Bartnicki-Garcia, S., and Nickerson, W. J. (1962a). Induction of yeastlike development in Mucor by carbon dioxide. J. Bacteriol. 84 829.

    Google Scholar 

  • Bartnicki-Garcia, S., and Nickerson, W. J. (1962b). Nutrition, growth, and morphogenesis of Mucor rouxii. J. Bacteriol. 84 841.

    Google Scholar 

  • Bonnichsen, R., and Lundgren, G. (1957). Comparison of the ADH and the Widmark procedures in forensic chemistry for determining alcohol. Acta Pharmacol. Toxicol. 13 256.

    Google Scholar 

  • Bulder, C. J. E. A. (1964). Induction of petite mutation and inhibition of synthesis of respiratory enzymes in various yeasts. Antonie van Leeuwenhoek J. Microbiol. Serol. 30 1.

    Google Scholar 

  • Ephrussi, B., Hottinguer, H., and Chiménes, A. M. (1949). Action de l'acriflavine sur les levures. I. La mutation “petite colonie.” Ann. Inst. Pasteur 76 351.

    Google Scholar 

  • Haidle, C. W., and Storck, R. (1966). Control of dimorphism in Mucor rouxii. J. Bacteriol. 92 1236.

    Google Scholar 

  • Hesseltine, C. W. (1954). The section genevensis of the genus Mucor. Mycologia 46 358.

    Google Scholar 

  • Langeron, M. (1945). Précis de Mycologie. Masson et Cie, (eds.), Libraires de L'Académie de Médecine, Paris.

    Google Scholar 

  • Layne, E. (1957). Spectrophotometric and turbidimetric methods for measuring proteins. In Colowick, S. P., and Kaplan, N. O. (eds.), Methods in Enzymology, vol. 3, Academic Press, New York, pp. 447–454.

    Google Scholar 

  • Lingappa, B. T., Presad, M., Lingappa, Y., Hunt, D. F., and Biemann, K. (1969). Phenethyl alcohol and triptophol: Autoantibiotics produced by the fungus Candida albicans. Science 163 192.

    Google Scholar 

  • Nagai, S., Yanagishima, N., and Nagai, H. (1961). Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms. Bacteriol. Rev. 25 404.

    Google Scholar 

  • Scheld, H. W., and Perry, J. J. (1970). Induction of dimorphism in the basidiomycete Lenzites saepiaria. J. Bacteriol. 102 271.

    Google Scholar 

  • Slonimski, P. P., Perrodin, G., and Croft, J. H. (1968). Ethidium bromide induced mutation of yeast mitochondria: Couple transformation of cells into respiratory deficient non-chromosomal “petites.” Biochem. Biophys. Res. Commun. 30 232.

    Google Scholar 

  • Smith, L. (1955). Spectrophotometric assay of cytochrome c oxidase. In Glick, D. (ed.), Methods of Biochemical Analysis, Vol. 2, Interscience, New York, pp. 427–434.

    Google Scholar 

  • Terenzi, H. F., and Storck, R. (1969a). Stimulation of fermentation and yeast-like morphogenesis in Mucor rouxii by phenethyl alcohol. J. Bacteriol. 97 1248.

    Google Scholar 

  • Terenzi, H. F., and Storck, R. (1969b). The effect of phenethyl alcohol on the morphology, growth, and metabolism of some mucorales. Mycologia 61 894.

    Google Scholar 

  • Turian, G. (1966). The genesis of macroconidia of Neurospora. In Madelin, M. F. (ed.), The Fungus Spore, Colston Papers No. 18, Butterworths, London, pp. 61–66.

    Google Scholar 

  • Umbreit, W. W., Burris, R. H., and Stauffer, J. F. (1957). Manometric Techniques, Burgess Publishing Co., Minneapolis.

    Google Scholar 

  • Wilkie, D., and Maroudas, N. G. (1969). Induction of cytoplasmic respiratory deficiency in yeast by phenethyl alcohol. Genet. Res. 13 107.

    Google Scholar 

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This investigation was supported by Public Health Service Grant AI 07804 05.

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Storck, R., Morrill, R.C. Respiratory-deficient, yeastlike mutant of Mucor . Biochem Genet 5, 467–479 (1971). https://doi.org/10.1007/BF00487136

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  • DOI: https://doi.org/10.1007/BF00487136

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