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Glucose 6-phosphate dehydrogenase from brook, lake, and splake trout: An isozymic and immunological study

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Abstract

There are five G6P-specific G6PD isozymes in both brook and lake trout. The most anodal isozyme in each species has the same electrophoretic mobility; however, the five lake trout isozymes are more widely spaced on polyacrylamide gels than are those from brook trout. In hybrids, i.e., splake trout, nine forms of G6PD can be resolved. These results can be explained by a model in which we assume that each isozyme is a tetramer and that two different subunit types are produced. In splake trout, three electrophoretically distinct subunits yield 15 tetramers. That only nine are detected is a consequence of coincident electrophoretic mobility of some of the possible subunit combinations. Our results indicate that the G6PD isozymes in these trout are the products of two codominant autosomal gene loci. The hypothesis that G6PD and H6PD arose from a common ancestral type G6PD is supported by microcomplement fixation data which show an immunochemical relatedness between these enzymes. The relationship of G6PD and H6PD in trout is discussed from an evolutionary standpoint.

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Author notes

  1. Toshio Yamauchi

    Present address: Department of Biology, The University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston, Houston, Texas

Authors and Affiliations

  1. Department of Biological Sciences, Northwestern University, Evanston, Illinois

    Toshio Yamauchi & Erwin Goldberg

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  1. Toshio Yamauchi
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  2. Erwin Goldberg
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Additional information

This research was supported in part by National Science Foundation Grant GB-7271 and by United States Public Health Predoctoral Fellowship 4 FO1 GM 43657-03.

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Yamauchi, T., Goldberg, E. Glucose 6-phosphate dehydrogenase from brook, lake, and splake trout: An isozymic and immunological study. Biochem Genet 10, 121–134 (1973). https://doi.org/10.1007/BF00485760

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  • DOI: https://doi.org/10.1007/BF00485760

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