Abstract
Studies on two variants of X-linked enzyme, G6PD, in several inbred and outbred strains of Drosophila melanogaster suggest that (1) there is dosage compensation at this locus; (2) males have 20–33% more activity than females, due to enzyme-deficient eggs in the latter; (3) outcrossing Drosophila strains results in a significant rise in G6PD specific activity in such a way as to suggest the presence of two or more nonlinked loci specific in their effect on G6PD activity (the effect is twice as great in males as it is in females); (4) there is less “A” enzyme than “B” enzyme activity/mg protein in males, but they are equal in females; (5) the presence or absence of X-linked regulators for G6PD could not be ascertained.
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References
Cock, A. G. (1964). Dosage compensation and sex-chromatin in non-mammals. Genet. Res. 5 354.
Dobzhansky, T. H. (1957). The X-chromosome in the larval salivary glands of hybrids Drosophila insularis × Drosophila tropicalis. Chromosoma 8 691.
Goldschmidt, R. B. (1955). Theoretical Genetics, University of California Press, Berkeley, pp. 356–358.
Kazazian, H. H., Jr., Young, W. J., and Childs, B. (1965). X-linked 6-phosphogluconate dehydrogenase in Drosophila: Subunit associations. Science 150 1601.
King, R. C. Personal communication.
Komma, D. J. (1966). Effect of sex transformation genes in glucose-6-phosphate dehydrogenase activity in Drosophila melanogaster. Genetics 54 497.
Komma, D. J. (1968). Glucose-6-phosphate dehydrogenase in Drosophila: A sex-influenced electrophoretic variant. Biochem. Genet. 1 229.
Lee, G. L. (1968). Dosage compensation as a developmental phenomenon in Drosophila. Genet. Res. 11 115.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 265.
Mukherjee, A. S., and Beermann, W. (1965). Synthesis of ribonucleic acid by the X-chromosome of Drosophila melanogaster and the problem of dosage compensation. Nature 207 785.
Muller, H. J. (1932). Further studies on the nature and causes of gene mutations. Proc. 6th Intern. Congr. Genet. 1 213.
Muller, H. J. (1948). Evidence of the precision of genetic adaptation. Harvey Lectures 43 165.
Muller, H. J., and Kaplan, W. D. (1966). The dosage compensation of Drosophila and mammals as showing the accuracy of the normal type. Genet. Res. 8 41.
Sofer, W. H., and Ursprung, M. (1967). Ontogeny of alcohol dehydrogenase in Drosophila melanogaster. Am. Zoologist 7 178.
Steele, M. W., Young, W. J., and Childs, B. (1968). Glucose 6-phosphate dehydrogenase in Drosophila melanogaster: Starch gel electrophoretic variation due to molecular instability. Biochem. Genet. 2 159.
Stern, C. (1960). Dosage compensation—Development of a concept and new facts. Can. J. Genet. Cytol. 2 105.
Young, W. J. (1966). X-linked electrophoretic variation in 6-phosphogluconate dehydrogenase. J. Heredity 57 58.
Young, W. J., Porter, J. E., and Childs, B. (1964). Glucose-6-phosphate dehydrogenase in Drosophila: X-linked electrophoretic variants. Science 143 140.
Young, W. J., Smith, S. G., and Childs, B. Unpublished data.
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Aided by National Institutes of Health grants HD 00004, HD00486, and GM 14155.
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Steele, M.W., Young, W.J. & Childs, B. Genetic regulation of glucose 6-phosphate dehydrogenase activity in Drosophila melanogaster . Biochem Genet 3, 359–370 (1969). https://doi.org/10.1007/BF00485720
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DOI: https://doi.org/10.1007/BF00485720