Abstract
A micro-scale (for protein of the order of 10−9 mole) peptide mapping method in thin-layer cellulose is reported. Peptide maps of tryptic digests from glucagon, from normal and variant β-chains of human hemoglobin, from human glucose 6-phosphate dehydrogenase, and from human phosphoglycerate kinase showed satisfactory resolution; thus this method can be used for characterizing the structure of variant enzymes and proteins of 10−9 molar quantity.
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This research was supported by research grant GM 15253 from the National Institutes of Health.
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Watanabe, S., Yoshida, A. Micro-scale peptide mapping method for identification of variant proteins. Biochem Genet 5, 541–547 (1971). https://doi.org/10.1007/BF00485672
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DOI: https://doi.org/10.1007/BF00485672