Abstract
By using three genetic markers, the asynchronous activation of parental alleles during embryonic development was studied on interspecific hybrids between the male brown trout and the female rainbow trout. The genetic markers used were lactate dehydrogenase (LDH) A2 as well as C subunits and alcohol dehydrogenase (ADH) subunits. Starch gel electrophoresis was utilized. The presence of LDH A2 subunits was detected from a very early stage of development in whole embryo extracts. However, up to a few days after hatching (63rd day), A2 subunits present were exclusively of the A2R type derived from the rainbow mother. The coexistence of the products of both parental alleles at this gene locus became evident only at the 70th day. The LDH C subunits which occur only in the retina of the eye as well as the ADH subunits which occur only in liver made their first appearances at the 95th and 150th days, respectively. At these dates, only the maternally derived rainbow subunits were detectable. It took another 40 days for the paternally derived brown subunits to make their appearance.
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Altman, P. L., and Dittmer, D. S. (eds.) (1962). Growth Including Reproduction and Morphological Development. Fed. Am. Soc. Exptl. Biol., Washington, D.C.
Blanco, A., and Zinkham, W. H. (1962). Lactate dehydrogenases in human testes. Science 139 601.
Blanco, A., Zinkham, W. H., and Kupchyk, L. (1964). Genetic control and ontogeny of lactate dehydrogenase in pigeon testes. J. Exptl. Zool. 156 137.
Brown, D. D., and Gurdon, J. B. (1966). Size distribution and stability of DNA-like RNA synthesized during development of anucleolate embryos of Xenopus laevis. J. Mol. Biol. 19 399.
Elsdale, T. R., Fischberg, M., and Smith, S. (1958). A mutation that reduces nucleolar number in Xenopus laevis. Exptl. Cell. Res. 14 642.
Goldberg, E. (1962). Lactic and malic dehydrogenases in human spermatozoa. Science 139 602.
Markert, C. L. (1964). Cellular differentiation—an expression of differential gene function. In Congenital Malformations, The International Medical Congress, New York, pp. 163–174.
Morrison, W. J., and Wright, J. E. (1966). Genetic analysis of three lactate dehydrogenase isozyme systems in trout: Evidence for linkage of genes coding subunits A and B. J. Exptl. Zool. 163 259.
Nakano, E., and Whiteley, A. H. (1965). Differentiation of multiple molecular forms of four dehydrogenases in the teleost, Oryzias latipes, studied by disc electrophoresis. J. Exptl. Zool. 159 (2): 167.
Ohno, S. (1967). Sex Chromosomes and Sex-linked Genes. Vol. I. Monographs on Endocrinology, Springer-Verlag, Berlin-Heidelberg-New York.
Shaw, C., and Koen, A. (1964). On the identity of “nothing dehydrogenase.” J. Histochem. Cytochem. 13 431.
Smithies, O. (1959). An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals. Biochem. J. 71 585.
Wallace, H., and Birnstiel, M. L. (1966). Ribosomal cistrons and the nucleolar organizer. Biochem. Biophys. Acta 114 296.
Wilson, A. C., Kaplan, N. O., Lenin, C., Pesce, A., Reichlin, M., and Allison, W. S. (1964). Evolution of lactic dehydrogenases. Fed. Proc. 23 1258.
Wright, D. A., and Moyer, F. H. (1966). Parental influences on lactate dehydrogenase in the early development of hybrid frogs in the genus Rana. J. Exptl. Zool. 163 215.
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In Duarte, this work was supported in part by PHS grant CA-05138 from the National Cancer Institute, U.S. Public Health Service.
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Hitzeroth, H., Klose, J., Ohno, S. et al. Asynchronous activation of parental alleles at the tissue-specific gene loci observed on hybrid trout during early development. Biochem Genet 1, 287–300 (1968). https://doi.org/10.1007/BF00485183
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DOI: https://doi.org/10.1007/BF00485183