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Interaction of the opaque-2 gene with starch-forming mutant genes on the synthesis of zein in maize endosperm

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Abstract

The combination of opaque-2 with starch-modified or starch-deficient mutants produced a cumulative and synergistic effect, respectively, in regulating zein synthesis. The double mutant, brittle-2 opaque-2, which almost completely prevented the synthesis of Z1 and Z2, had high RNase activity. The possible involvement of RNase in effecting zein synthesis is discussed.

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References

  • Akatsuka, T., and Nelson, O. E. (1965). Starch granule-bound adenosine diphosphate glucose-starch glucosyltransferase of maize seeds. J. Biol. Chem. 2412280.

    Google Scholar 

  • Association of Official Agricultural Chemists (1970). Official Method of Analysis. 11th ed., AOAC, Washington, DC., p. 858.

    Google Scholar 

  • Burr, B., and Burr, F. A. (1976). Zein synthesis in maize endosperm by polyribosomes attached to protein bodies. Proc. Natl. Acad. Sci. 73515.

    Google Scholar 

  • Burr, B., and Nelson, O. E. (1973). The phosphorylases of developing maize seeds. Ann. N. Y. Acad. Sci. 210129.

    Google Scholar 

  • Cagampang, G. B., and Dalby, A. (1972). Development of ribonuclease activity in nine inbred lines of normal and opaque-2 maize. Can. J. Plant Sci. 52901.

    Google Scholar 

  • Chourey, P. S., and Nelson, O. E. (1976). The enzymatic deficiency conditioned by the shrunken-1 mutation in maize. Biochem. Genet. 141041.

    Google Scholar 

  • Creech, R. G. (1965). Genetic control of carbohydrate synthesis in maize endosperm. Genetics 511175.

    Google Scholar 

  • Dalby, A. (1974). Rapid method for determining the zein content of whole maize seed or isolated endosperm. Cereal Chem. 51586.

    Google Scholar 

  • Dalby, A., and Davies, I. (1967). Ribonuclease activity in the developing seed of normal and opaque-2 maize. Science 1551573.

    Google Scholar 

  • Dalby, A., and Tsai, C. Y. (1974). Zein accumulation in phenotypically modified lines of opaque-2. Cereal Chem. 51821.

    Google Scholar 

  • Dalby, A., and Tsai, C. Y. (1975). Comparisons of lysine and zein and non-zein protein contents in immature and mature maize endosperm mutants. Crop Sci. 15513.

    Google Scholar 

  • DiFonzo, N., Fornasari, E., Salamini, F., and Soave, C. (1977). SDS-protein subunits in normal, opaque-2 and floury-2 maize endosperms. Maydica 2277.

    Google Scholar 

  • Glover, D. V., Crane, P. L., Misra, P. S., and Mertz, E. T. (1975). Genetics of endosperm mutants in maize as related to protein quality and quantity. In High-Quality Protein Maize, Dowden, Hutchison and Ross, Inc., Stroudsburg, Pa., p. 228.

    Google Scholar 

  • Hannah, L. C., and Nelson, O. E. (1975). Characterization of adenosine diphosphate glucose pyrophosphorylases from developing maize seeds. Plant Physiol. 55297.

    Google Scholar 

  • Hansel, L. W., Tsai, C. Y., and Nelson, O. E. (1973). The effect of the floury-2 gene on the distribution of protein fractions and methionine in maize endosperm. Cereal Chem. 50383.

    Google Scholar 

  • Jones, R. A., Dalby, A., and Tsai, C. Y. (1975). A note on a modified technique for the rapid determination of zein content in maize. Cereal Chem. 52279.

    Google Scholar 

  • Jones, R. A., Larkins, B. A., and Tsai, C. Y. (1977a). Storage protein synthesis in maize. II. Reduced synthesis of a major zein component by the opaque-2 mutant of maize. Plant Physiol. 59525.

    Google Scholar 

  • Jones, R. A., Larkins, B. A., and Tsai, C. Y. (1977b). Storage protein synthesis in maize. III. Developmental changes in membrane-bound polyribosome composition and in vitro protein synthesis of normal and opaque-2 maize. Plant Physiol. 59733.

    Google Scholar 

  • Laemmli, U. K. (1970). Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227680.

    Google Scholar 

  • Larkins, B. A., and Dalby, A. (1975). In vitro synthesis of zein-like protein by maize polyribosomes. Biochem. Biophys. Res. Commun. 661042.

    Google Scholar 

  • Larkins, B. A., Bracker, C. E., and Tsai, C. Y. (1976). Storage protein synthesis in maize. Isolation of zein-synthesizing polyribosomes. Plant Physiol. 57740.

    Google Scholar 

  • Lee, K. H., Jones, R. A., Dalby, A., and Tsai, C. Y. (1976). Genetic regulation of storage protein synthesis in maize endosperm. Biochem. Genet. 14641.

    Google Scholar 

  • Marcu, K., and Dudock, B. (1974). Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acid Res. 11385.

    Google Scholar 

  • Mertz, E. T., Bates, L. S., and Nelson, O. E. (1964). Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145279.

    Google Scholar 

  • Misra, P. S., Jamunathan, R., Mertz, E. T., Glover, D. V., Barbosa, H. M., and McWhirter, K. S. (1972). Endosperm protein synthesis in maize mutants with increased lysine content. Science 1761425.

    Google Scholar 

  • Misra, P. S., Mertz, E. T., and Glover, D. V. (1975). Studies on corn proteins. VI. Endosperm protein changes in single and double endosperm mutants of maize. Cereal Chem. 52161.

    Google Scholar 

  • Misra, P. S., Mertz, E. T., and Glover, D. V. (1976). Studies on corn proteins. X. Polypeptide molecular-weight distribution in Landry-Moreaux fractions of normal and mutant endosperms. Cereal Chem. 53705.

    Google Scholar 

  • Nelson, O. E., Mertz, E. T., and Bates, L. S. (1965). Second mutant gene affecting the amino acid pattern of maize endosperm proteins. Science 1501469.

    Google Scholar 

  • Palmiter, R. D. (1974). Differential rates of initiation on conalbumin and ovalbumin messenger ribonucleic acid in reticulocyte lysates. Biochemistry 133606.

    Google Scholar 

  • Paulis, J. W., Bietz, J. A., and Wall, J. S. (1975). Corn protein subunits: Molecular weights determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J. Agr. Food Chem. 23197.

    Google Scholar 

  • Roe, J. H. (1934). A colorimetric method for the determination of fructose in blood and urine. J. Biol. Chem. 10715.

    Google Scholar 

  • Soave, C., Righetti, P. G., Lorenzoni, c., Gentinetta, E., and Salamini, F. (1976). Expressivity of the opaque-2 gene at the level of zein molecular components. Maydica 2161.

    Google Scholar 

  • Tsai, C. Y. (1974). The function of the waxy locus in starch synthesis in maize endosperm. Biochem. Genet. 1183.

    Google Scholar 

  • Tsai, C. Y., and Dalby, A. (1974). Comparisons of the effect of shrunken-4, opaque-2, opaque-7 and floury-2 genes on the zein content of maize during endosperm development. Cereal Chem. 51825.

    Google Scholar 

  • Tsai, C. Y., and Nelson, O. E. (1966). Starch-deficient maize mutant lacking adenosine diphosphate glucose pyrophosphorylase activity. Science 151341.

    Google Scholar 

  • Tsai, C. Y., Salamini, F., and Nelson, O. E. (1970). Enzymes of carbohydrate metabolism in the developing endosperm of maize. Plant Physiol. 46299.

    Google Scholar 

  • Tuve, T. W., and Anfinsen, C. B. (1960). Preparation and properties of spinach ribonuclease. J. Biol. Chem. 2353437.

    Google Scholar 

  • Weaver, S. H., Glover, D. V., and Tsai, C. Y. (1972). Nucleoside diphosphate glucose pyrophosphorylase isozymes of developing normal, brittle-2 and shrunken-2 endosperms of Zea mays L. Crop Sci. 12510.

    Google Scholar 

  • Wilson, C. M. (1963). Chromatographic separation of ribonucleases in corn. Biochim. Biophys. Acta 68177.

    Google Scholar 

  • Wilson, C. M., and Alexander, D. E. (1967). Ribonuclease activity in normal and opaque-2 mutant endosperm of maize. Science 1551575.

    Google Scholar 

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This work was supported in part by the Lilly Endowment and the Agency for International Development under contract “Inheritance and Improvement of Protein Quality and Content in Maize.”

Journal Paper no. 6968, Purdue University Agricultural Experiment Station.

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Tsai, C.Y., Larkins, B.A. & Glover, D.V. Interaction of the opaque-2 gene with starch-forming mutant genes on the synthesis of zein in maize endosperm. Biochem Genet 16, 883–896 (1978). https://doi.org/10.1007/BF00483740

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  • DOI: https://doi.org/10.1007/BF00483740

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