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PrÄchromatographische in situ-Derivatisierung von FettsÄuren im Picomol-Bereich

Teil I: HPTLC fluorescierender Monodansylpiperizin- und -cadaverin-Derivate

Prechromatographic in situ-derivatization of fatty acids in the picomole range

Part I: HPTLC of fluorescent monodansylpiperazine- and monodansylcadaverine-derivatives

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Summary

A simple and rapid derivatization method for C24-C6 fatty acids on HPTLC RP-18 phases is described. The prechromatographic reaction with monodansylpiperazine and monodansylcadaverine takes place at the start. Linear calibration curves are obtained after chromatography (e.g. dansyl palmityl piperazide: r=0.9998), which can be employed for quantitative analysis in the lower nanogram range (determination limit <10 ng). All 10 fatty acids can be separated excellently (R usually 1.5). Stepwise and gradient developments are employed (AMD system).

This is the first time that in situ derivatization techniques have been employed for fatty acids leading to fluorescent products in the picomole range and a gradient method has been described which also leads to precise separations on RP phases.

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Literatur

  1. Farag RS, El-Sharabassy AAM, Abdel-Rahim EA, Hewedy FM, Ragab AA (1986) Seifen, öle, Fette, Wachse 112:453–455

    Google Scholar 

  2. Fabian C, Scheppach W, Kasper H (1987) Ernaehrungsumschau 34:94

    Google Scholar 

  3. Leichsenring M, Bremer HJ (1987) Ernaehrungsumschau 34:99

    Google Scholar 

  4. Tsuchiya H, Hayashi T, Sato M, Tatsumi M, Tagaki N (1984) J Chromatogr 309:43–52

    Google Scholar 

  5. Yanagisawa I, Yamane M, Urayama T (1985) J Chromatogr 345:229–240

    Google Scholar 

  6. Gloor R, Leidner H (1976) Chromatographia 9:618–623

    Google Scholar 

  7. Ziolecki A, Kwiatkowska E (1973) J Chromatogr 80:250–254

    Google Scholar 

  8. Du Preez IC, Lategan PM (1978) J Chromatogr 150:259–262

    Google Scholar 

  9. Larsson M, Roos C (1983) Chromatographia 17:185–190

    Google Scholar 

  10. Barcelona MJ, Liljestrand HM, Morgan JJ (1980) Anal Chem 52:321–325

    Google Scholar 

  11. Patience RL, Thomas JD (1982) J Chromatogr 234:225–230

    Google Scholar 

  12. L'Emeillat Y, Ménez JF, Berthou F, Bardou L (1981) J Chromatogr 206:89–100

    Google Scholar 

  13. Umeh EO (1971) J Chromatogr 56:29–36

    Google Scholar 

  14. Chauhan J, Darbre A (1982) J Chromatogr 240:107–115

    Google Scholar 

  15. Kim K, Zlatkis A, Horning EC, Middleditch BS (1987) J High Res Chromatogr Chromatogr Comm 10:522–523

    Google Scholar 

  16. Haniš T, SmrŽ M, Klír P, Macek K, Deyl Z (1986) Collect Czech Chem Commun 51:2722–2726

    Google Scholar 

  17. Engelhardt H, Elgass H (1978) J Chromatogr 158:249–259

    Google Scholar 

  18. Durst HD, Milano M, Kikta EJ Jr, Connelly SA, Grushka E (1975) Anal Chem 47:1797–1801

    Google Scholar 

  19. Borch RF (1975) Anal Chem 47:2437–2439

    Google Scholar 

  20. Osterroht C (1987) Chromatographia 23:419–422

    Google Scholar 

  21. Halgunset J, Lund EW, Sunde A (1982) J Chromatogr 237:496–499

    Google Scholar 

  22. Netting AG, Duffield AM (1984) J Chromatogr 336:115–123

    Google Scholar 

  23. Hoffman NE, Liao JC (1976) Anal Chem 48:1104–1106

    Google Scholar 

  24. Miwa H, Hiyam C, Yamamoto M (1985) J Chromatogr 321:165–174

    Google Scholar 

  25. Cooper MJ, Anders MW (1974) Anal Chem 46:1849–1852

    Google Scholar 

  26. Gübitz G (1980) J Chromatogr 187:208–211

    Google Scholar 

  27. Lam S, Grushka E (1978) J Chromatogr 158:207–214

    Google Scholar 

  28. Ikeda M, Shimada K, Sakaguchi T, Matsumoto U (1984) J Chromatogr 305:261–270

    Google Scholar 

  29. Lloyd JBF (1980) J Chromatogr 189:359–373

    Google Scholar 

  30. Ryan PJ, Honeyman TW (1984) J Chromatogr 312:416–466

    Google Scholar 

  31. Kany E, Junker-Buchheit A, Jork H (1986) GDCh-Fortbildungskurs Nr. 300, Saarbrücken

  32. Haeffner EW (1970) Lipids 5:430–433

    Google Scholar 

  33. Nicolosi RJ, Smith SC, Santerre RF (1971) J Chromatogr 60:111–117

    Google Scholar 

  34. Knappe E, Peteri D (1962) Fresenius Z Anal Chem 188:184–189

    Google Scholar 

  35. Kany E, Jork H (1985) GDCh-Fortbildungskurs Nr. 300, Saarbrücken

  36. AnfÄrbereagentien für Dünnschicht- und Papier-Chromatographie, E Merck, Darmstadt 1970

  37. Gattavecchia E, Tonelli D, Bertocchi G (1983) J Chromatogr 260:517–521

    Google Scholar 

  38. Dünges W (1977) Anal Chem 49:442–445

    Google Scholar 

  39. HÄnsel W, Strömmer R (1987) GIT Fachz Lab Suppl Chromatogr 31:21–26

    Google Scholar 

  40. Jork H, Funk W, Fischer W, Wimmer H (1988) Dünnschicht-Chromatographie: Reagenzien und Nachweismethoden, Bd 1a. VCH, Verlagsgesellschaft, Weinheim

    Google Scholar 

  41. Dünges W (1982) GIT Fachz Lab Suppl Chromatogr 26:17–26

    Google Scholar 

  42. Funk W (1984) Fresenius Z Anal Chem 318:206–219

    Google Scholar 

  43. Burger K (1984) Fresenius Z Anal Chem 318:228–233

    Google Scholar 

  44. Burger K (1984) GIT Fachz Lab Suppl Chromatogr 28:29–31

    Google Scholar 

  45. JÄnchen DE (1986) LP-Spezial „Chromatographie & Spektroskopie“. GIT-Verlag, Darmstadt, S 104–109

    Google Scholar 

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Authors and Affiliations

  1. Fachbereich Pharmazie und Biologische Chemie, UniversitÄt des Saarlandes, D-6600, Saarbrücken, Bundesrepublik Deutschland

    A. Junker-Buchheit & H. Jork

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  1. A. Junker-Buchheit
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  2. H. Jork
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Herrn Prof. Dr. W. Fresenius zu seinem 75. Geburtstag in Dankbarkeit gewidmet

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Junker-Buchheit, A., Jork, H. PrÄchromatographische in situ-Derivatisierung von FettsÄuren im Picomol-Bereich. Z. Anal. Chem. 331, 387–393 (1988). https://doi.org/10.1007/BF00481914

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  • DOI: https://doi.org/10.1007/BF00481914

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