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Reaktivierbare Enzymelektrode mit Acetylcholinesterase zur differenzierten Erfassung von Insecticiden im Spurenbereich

Reactivatable enzyme electrode with acetylcholine-esterase for the differentiated detection of insecticides in the trace range

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Summary

The enzyme acetylcholine-esterase is immobilised in a matrix of bovine albumine by crosslinking with glutaraldehyde directly at the surface area of a pH electrode. A reversible inhibition by methyl carbamate insecticides is detectable from 0.1 mg/l (Aldicarb), a irreversible inhibition is detectable by phosphoric acid ester insecticides from 50 Μg/l (Dichlorvos). After oxidation by bromine water also phosphoric acid thioester insecticides (Parathionethyl) are detectable from 2.5 Μg/1. A reactivation of the enzyme electrode by means of pyridine-2-aldoximemethyliodide as well as an analysis scheme for differentiated detection of the three insecticide groups in water and on fruits and vegetables are demonstrated.

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Author notes

  1. Georg Schwedt

    Present address: Institut für Anorganische und Analytische Chemie, TU Clausthal, Paul-Ernst-Strasse 4, D-3392, Clausthal-Zellerfeld, Bundesrepublik Deutschland

Authors and Affiliations

  1. Institut für Lebensmittelchemie und Analytische Chemie der UniversitÄt Stuttgart, Pfaffenwaldring 55, D-7000, Stuttgart 80, Bundesrepublik Deutschland

    Georg Schwedt & Michael Hauck

Authors
  1. Georg Schwedt
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  2. Michael Hauck
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Additional information

Herrn Prof. Dr. Wilhelm Fresenius zum 75. Geburtstag gewidmet

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Schwedt, G., Hauck, M. Reaktivierbare Enzymelektrode mit Acetylcholinesterase zur differenzierten Erfassung von Insecticiden im Spurenbereich. Z. Anal. Chem. 331, 316–320 (1988). https://doi.org/10.1007/BF00481902

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  • DOI: https://doi.org/10.1007/BF00481902

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