Conclusions
The proposed method offers the following advantages:
a) high reactivity of the enzyme; b) need for small sample volume, thus absorption from suspended particles does not interfere and c) nearly complete solubilization of particle bound enzyme thus allowing automated analysis with the supernatant after centrifugation.
References
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Haverback BJ, Dyce BJ, Gutenberg PJ, Montgomery DW (1963) Gastroenterology 44:588–597
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Kaspar, P., Moeller, G., Wahlefeld, A.W. et al. A new photometric method for the determination of chymotrypsin in stool. Z. Anal. Chem. 311, 391–392 (1982). https://doi.org/10.1007/BF00481761
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DOI: https://doi.org/10.1007/BF00481761