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Deoxyribonucleic acid reassociation in the taxonomy of the genus Chlorella

I. Chlorella sorokiniana

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Abstract

Two different DNA hybridization methods were used to study the genetic relationships among strains of Chlorella sorokiniana. Closely related strains could be easily detected by comparison of DNA renaturation rates as determined spectrophotometrically, whereas the degree of relationship of more remotely related strains could be determined by means of reassociation of radioactively labelled DNA fragments with high-molecular-weight DNA bound to nitrocellulose filters. Strains assigned to C. sorokiniana proved to be a rather heterogeneous group with only 3 out of 17 strains being closely related to the type strain C. sorokiniana 211-8kT. The description of new species or subspecific taxa on the basis of nucleotide sequence similarities, however, does not seem justified before more detailed knowledge of genome organization in Chlorella is available.

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Abbreviations

1xSSC:

standard saline citrate (0.15 M NaCl+0.015 M trisodium citrate, pH 7.0)

Tris:

tris-(hydroxymethyl)-aminomethane

EDTA:

ethylene diamine tetraacetate

CTAB:

N-cetyl-N,N,N-trimethyl ammonium bromide

TEACl:

tetraethyl ammonium chloride

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Authors and Affiliations

  1. Institut für Botanik und Pharmazeutische Biologie der Universität, Staudtstr. 5, D-8520, Erlangen, Federal Republic of Germany

    Volker A. R. Huss, Richard Dörr, Ulrike Grossmann & Erich Kessler

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  1. Volker A. R. Huss
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  2. Richard Dörr
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  3. Ulrike Grossmann
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  4. Erich Kessler
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Huss, V.A.R., Dörr, R., Grossmann, U. et al. Deoxyribonucleic acid reassociation in the taxonomy of the genus Chlorella . Arch. Microbiol. 145, 329–333 (1986). https://doi.org/10.1007/BF00470866

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  • DOI: https://doi.org/10.1007/BF00470866

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