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A graphics display-oriented strategy for the amino acid sequencing of peptides by tandem mass spectrometry

Bestimmung der Aminosäure-Sequenz von Peptiden durch Tandem-Massenspektrometrie mit Hilfe eines graphischen Display

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Summary

A graphics display-oriented method for the computer-aided interpretation of the mass spectrum of a peptide in terms of its amino acid sequence is presented. The spectrum is obtained by collision induced decomposition of the protonated molecular ion (M+H)+ of the peptide generated by fast atom bombardment in the first double focussing mass analyzer of a tandem mass spectrometer and the product ion mass spectrum (CID spectrum) recorded by the second double focussing mass analyzer. The algorithm displays all series of peaks differing by the mass of -NH-CHR-CO- for the various amino acids. This display, which is very fast and can be augmented through interrogation by the user, greatly facilitates the determination of the amino acid sequence. The method is demonstrated on the spectra of a undecapeptide of (M+H)+ m/z = 1208.2 and a tetradecapeptide of (M+H)+ m/z = 1758.9.

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Author notes

  1. Hubert A. Scoble

    Present address: Genetics Institute, 87 Cambridge Park Dr., 02140, Cambridge, MA, USA

Authors and Affiliations

  1. Department of Chemistry (56-010), Massachusetts Institute of Technology, 02139, Cambridge, MA, USA

    Hubert A. Scoble, James E. Biller & Klaus Biemann

Authors
  1. Hubert A. Scoble
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  2. James E. Biller
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  3. Klaus Biemann
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Scoble, H.A., Biller, J.E. & Biemann, K. A graphics display-oriented strategy for the amino acid sequencing of peptides by tandem mass spectrometry. Z. Anal. Chem. 327, 239–245 (1987). https://doi.org/10.1007/BF00469824

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  • DOI: https://doi.org/10.1007/BF00469824

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